| Antigen detectable with this ELISA kit: |
Protocadherin 15 (PCDH15) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
065ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protocadherin 15 (PCDH15), The concentration of Protocadherin 15 (PCDH15) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protocadherin 15 (PCDH15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Protocadherin 15 (PCDH15), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Protocadherin 15 (PCDH15) |
| Short name: |
Protocadherin 15 (PCDH15) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Protocadherin 15 (protocadherin-related 15) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, PCDH15 and IDBG-637041 and ENSBTAG00000045905 and 100140108, PCDH15 and IDBG-74763 and ENSG00000150275 and 65217, Pcdh15 and IDBG-162009 and ENSMUSG00000052613 and 11994, protein binding, this GO :0001750 and photoreceptor outer segment and cellular component this GO :0001964 and startle response and biological process this GO :0002009 and morphogenesis of an epithelium and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007015 and actin filament organization and biological process this GO :0007156 and homophilic cell adhesion and biological process this GO :0007601 and visual perception and biological process this GO :0007605 and sensory perception of sound and biological process this GO :0007626 and locomotory behavior and biological process this GO :0007628 and adult walking behavior and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0032420 and stereocilium and cellular component this GO :0035058 and nonmotile primary cilium assembly and biological process this GO :0035264 and multicellular organism growth and biological process this GO :0042491 and auditory receptor cell differentiation and biological process this GO :0045202 and synapse and cellular component this GO :0045494 and photoreceptor cell maintenance and biological process this GO :0048839 and inner ear development and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0050953 and sensory perception of light stimulus and biological process this GO :0050957 and equilibrioception and biological process this GO :0050973 and detection of mechanical stimulus involved in equilibrioception and biological process this GO :0060013 and righting reflex and biological process this GO :0060088 and auditory receptor cell stereocilium organization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005509 : calcium ion binding, this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, protocadherin-related 15 |
| Identity: |
14674 |
| Gene: |
PCDH15 |
More about : PCDH15 |
| Long gene name: |
protocadherin related 15 |
| Synonyms gene: |
USH1F DFNB23 |
| Synonyms gene name: |
autosomal recessive 23 protocadherin 15 , deafness |
| Synonyms: |
CDHR15 |
| Synonyms name: |
cadherin-related family member 15 |
| Locus: |
10q21, 1 |
| Discovery year: |
2001-02-27 |
| GenBank acession: |
AY029205 |
| Entrez gene record: |
65217 |
| Pubmed identfication: |
11398101 14570705 |
| RefSeq identity: |
NM_033056 |
| Classification: |
Cadherin related Deafness associated genes |
| Havana BLAST/BLAT: |
OTTHUMG00000018259 |
| Locus Specific Databases: |
Mutations of the Protocadherin 15 Gene CCHMC-BMI &, UC Hearing Loss Mutation Database |