| We send this reagant on: |
Room temperature |
| The temperatureerature for storage is: |
Avoid multiple freeze/thaw cycles, Store the store the remaining components at 4º, store the DCFH-DA Probe (1000X) and Quercetin at -20º, C, C, Upon receipt |
| Technical file: |
more kit, pdf |
| SDS or safty data sheet: |
details (STA-349) GHS, pdf |
| Type of reagent: |
Oxidative Stress/Damage |
| Related techniques: |
Antioxidant Assays, Cellular Antioxidant Assay, Oxidative Stress/ Damage |
| Use of the reagent: |
After a brief incubation, Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent, Cellular Antioxidant Assay test kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells, The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF, The Quercetin inhibits the formation of free radicals, Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, and the reaction started by adding the Free Radical Initiator, and thus DCF formation, in a concentration dependent manner, or the antioxidant sample being tested, the cells are washed, , , , , The OxiSelect&trade |
| Cellular use: |
Determine the efficacy of an exogenous antioxidant compound within a cellular environment |
| Protocol information: |
including standard curve and unknown samples, Each test kit provides sufficient reagents to perform up to 192 assays |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
7, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Test: |
Cellular biology labs supplies other types of Assays as 1 |
| Gene target: |
Cellular Antioxidant test kit, OxiSelect&trade |
| Short name: |
Cellular Antioxidant Assay test kit ( Fluorescence), OxiSelect&trade |
| Technique: |
assays, Assay |
| Host: |
Assay |
| Alternative name: |
Cellular Antioxidant test test reagent (Green Fluorescence), OxiSelect&trade |
| Alternative technique: |
arrays |