| We send this reagant on: |
Room temperature |
| The temperatureerature for storage is: |
Avoid multiple freeze/thaw cycles, DCF and Hydrogen Peroxide at -20º, Store the Cell Lysis Buffer at 4º, store the DCFH-DA, C, C, Upon receipt |
| Technical file: |
more kit, pdf |
| SDS or safty data sheet: |
details (STA-342) GHS, pdf |
| Type of reagent: |
Oxidative Stress/Damage |
| Related techniques: |
Intracellular ROS Assay, Reactive Oxygen Species (ROS) Assays, Oxidative Stress/ Damage |
| Use of the reagent: |
DCFH is rapidly oxidized to highly fluorescent DCF, Fluorescence is read on , In the presence of ROS, ROS Assay test kit is a cell-based assay for measuring hydroxyl, The assay employs the cell-permeable fluorogenic probe DCFH-DA, and other reactive oxygen species activity within a cell, peroxyl, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1), The OxiSelect&trade, a standard Fluorimetry plate reader |
| Cellular use: |
Quick , ~1 hour protocol |
| Protocol information: |
Highly sensitive to 10 pM |
| Reagent properties: |
Detects the presence of various ROS species |
| Living modified organisme in this reagent or LMO: |
Not applicable |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
89, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Test: |
Cellular biology labs supplies other types of Assays as 1 |
| Gene target: |
Intracellular ROS test kit, OxiSelect&trade |
| Short name: |
Intracellular ROS Assay test kit ( Fluorescence), OxiSelect&trade |
| Technique: |
assays, Assay |
| Host: |
Assay |
| Alternative name: |
Intracellular ROS test test reagent (Green Fluorescence), OxiSelect&trade |
| Alternative technique: |
arrays |
| Identity: |
10261 |
| Gene: |
ROS1 |
More about : ROS1 |
| Long gene name: |
ROS proto-oncogene 1, receptor tyrosine kinase |
| Synonyms gene name: |
receptor tyrosine kinase , v-ros avian UR2 sarcoma virus oncogene homolog 1 v-ros UR2 sarcoma virus oncogene homolog 1 (avian) c-ros oncogene 1 |
| Synonyms: |
MCF3 ROS c-ros-1 |
| Locus: |
6q22, 1 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M13599 |
| Entrez gene record: |
6098 |
| Pubmed identfication: |
1611909 |
| Classification: |
Fibronectin type III domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000016188 |
| Locus Specific Databases: |
LRG_997 |