| We send this reagant on: |
Blue Ice |
| The temperatureerature for storage is: |
-20º, C |
| SDS or safty data sheet: |
more (STA-321-T) GHS, pdf |
| Type of reagent: |
Oxidative Stress/Damage |
| Related techniques: |
DNA / RNA Damage and Repair, DNA Double-Strand Break Assay, Oxidative Stress/ Damage |
| Use of the reagent: |
DNA Double-Strand Break Staining test kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates, Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX, H2AX, One of the earliest cellular responses to double-strand breaks , Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites, The , Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells, at the sites of DNA damage, Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells, OxiSelect&trade, is the phosphorylation of a histone variant |
| Cellular use: |
Detects phosphorylation of histone , H2A, X |
| Protocol information: |
See immunofluorescence in about 3 hours |
| Reagent properties: |
DNA , double-strand break , inducer included |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
51, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Gene target: |
DNA Double Strand Break (DSB) Staining test kit, Trial Size, OxiSelect&trade |
| Short name: |
DNA Double Strand Break (DSB) Staining test kit, Trial Size, OxiSelect&trade |
| Alternative name: |
Desoxyribonucleic acid Double Strand Break (DSB) coloring test reagent, Trial Size, OxiSelect&trade |
| Alternative technique: |
tests |