| Antigen detectable with this ELISA kit: |
Protein Disulfide Isomerase A2 (PDIA2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
113ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2 (PDIA2), The concentration of Protein Disulfide Isomerase A2 (PDIA2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2 (PDIA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Protein Disulfide Isomerase A2 (PDIA2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Protein Disulfide Isomerase A2 (PDIA2) |
| Short name: |
Protein Disulfide Isomerase A2 (PDIA2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
member 2) Enzyme-linked immunosorbent assay reagent, Protein Disulfide Isomerase A2 (protein disulfide isomerase family A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, PDIA2 and IDBG-7384 and ENSG00000185615 and 64714, Pdia2 and IDBG-154856 and ENSMUSG00000024184 and 69191, member 2, multiple, peptidyl-proline 4-dioxygenase activity, this GO :0003756 and protein disulfide isomerase activity and molecular function this GO :0005496 and steroid binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0006457 and protein folding and biological process this GO :0006621 and protein retention in ER lumen and biological process this GO :0008152 and metabolic process and biological process this GO :0016209 and antioxidant activity and molecular function this GO :0016491 and oxidoreductase activity and molecular function this GO :0016853 and isomerase activity and molecular function this GO :0019511 and peptidyl-proline hydroxylation and biological process this GO :0031545 and peptidyl-proline 4-dioxygenase activity and molecular function this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :1902175 and regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process, this GO :0003756 : protein disulfide isomerase activity, this GO :0003756 : protein disulfide isomerase activity and also this GO :0005496 : steroid binding and also this GO :0005515 : protein binding and also this GO :0016209 : antioxidant activity and also this GO :0016491 : oxidoreductase activity and also this GO :0016853 : isomerase activity and also this GO :0031545 : peptidyl-proline 4-dioxygenase activity, this GO :0005496 : steroid binding, this GO :0005515 : protein binding, this GO :0016209 : antioxidant activity, this GO :0016491 : oxidoreductase activity, this GO :0016853 : isomerase activity, this GO :0031545 : peptidyl-proline 4-dioxygenase activity, 51496 and IDBG-633666 and ENSBTAG00000016575 and 504356, protein disulfide isomerase family A |
| Identity: |
14180 |
| Gene: |
PDIA2 |
More about : PDIA2 |
| Long gene name: |
protein disulfide isomerase family A member 2 |
| Synonyms gene: |
PDIP |
| Synonyms gene name: |
member 2 , pancreatic protein disulfide isomerase-associated 2 protein disulfide isomerase family A, protein disulfide isomerase |
| Synonyms: |
PDA2 PDI PDIR |
| Locus: |
16p13, 3 |
| Discovery year: |
2000-12-21 |
| GenBank acession: |
U19948 |
| Entrez gene record: |
64714 |
| Pubmed identfication: |
8561901 |
| RefSeq identity: |
NM_006849 |
| Classification: |
Protein disulfide isomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000064891 |