| Antigen detectable with this ELISA kit: |
Platelet Factor 4 (PF4) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25pg/mL, 50pg/mL, 125pg/mL, 25pg/mL, 563-100pg/mL The standard curve concentrations used for the ELISA&rsquo, 563pg/mL, 5pg/mL, s were 100pg/mL |
| Sensitivity: |
64pg/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
platelet-poor plasma and cell culture supernates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4), The concentration of Platelet Factor 4 (PF4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Platelet Factor 4 (PF4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Platelet Factor 4 (PF4) |
| Short name: |
Platelet Factor 4 (PF4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Platelet Factor 4 (platelet factor 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CXCL4 and PF-4 and SCYB4, CXCR3 chemokine receptor binding, Extracellular, PF4 and IDBG-24097 and ENSG00000163737 and 5196, this GO :0002576 and platelet degranulation and biological process this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0007596 and blood coagulation and biological process this GO :0008009 and chemokine activity and molecular function this GO :0008201 and heparin binding and molecular function this GO :0010628 and positive regulation of gene expression and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030168 and platelet activation and biological process this GO :0030595 and leukocyte chemotaxis and biological process this GO :0030816 and positive regulation of cAMP metabolic process and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043950 and positive regulation of cAMP-mediated signaling and biological process this GO :0045347 and negative regulation of MHC class II biosynthetic process and biological process this GO :0045651 and positive regulation of macrophage differentiation and biological process this GO :0045653 and negative regulation of megakaryocyte differentiation and biological process this GO :0045918 and negative regulation of cytolysis and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048248 and CXCR3 chemokine receptor binding and molecular function this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0008009 : chemokine activity, this GO :0008009 : chemokine activity and also this GO :0008201 : heparin binding and also this GO :0048248 : CXCR3 chemokine receptor binding, this GO :0008201 : heparin binding, this GO :0048248 : CXCR3 chemokine receptor binding, platelet factor 4 |
| Identity: |
8861 |
| Gene: |
PF4 |
More about : PF4 |
| Long gene name: |
platelet factor 4 |
| Synonyms gene name: |
platelet factor 4 |
| Synonyms: |
SCYB4 CXCL4 |
| Synonyms name: |
chemokine (C-X-C motif) ligand 4 |
| Locus: |
4q13, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M25897 |
| Entrez gene record: |
5196 |
| Pubmed identfication: |
3622011 |
| Havana BLAST/BLAT: |
OTTHUMG00000130009 |