| Antigen detectable with this ELISA kit: |
Platelet Derived Growth Factor Subunit B (PDGFB) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
3pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB), The concentration of Platelet Derived Growth Factor Subunit B (PDGFB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Platelet Derived Growth Factor Subunit B (PDGFB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Platelet Derived Growth Factor Subunit B (PDGFB) |
| Short name: |
Platelet Derived Growth Factor Subunit B (PDGFB) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Platelet Derived Growth Factor functionnal sequence B (platelet-derived growth factor b polypeptide) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048008 and platelet-derived growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048407 and platelet-derived growth factor binding and molecular function this GO :0048514 and blood vessel morphogenesis and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0050730 and regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0050921 and positive regulation of chemotaxis and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060445 and branching involved in salivary gland morphogenesis and biological process this GO :0060664 and epithelial cell proliferation involved in salivary gland morphogenesis and biological process this GO :0061098 and positive regulation of protein tyrosine kinase activity and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :0071506 and cellular response to mycophenolic acid and biological process this GO :0072126 and positive regulation of glomerular mesangial cell proliferation and biological process this GO :0072255 and metanephric glomerular mesangial cell development and biological process this GO :0072262 and metanephric glomerular mesangial cell proliferation involved in metanephros development and biological process this GO :0072264 and metanephric glomerular endothelium development and biological process this GO :0072593 and reactive oxygen species metabolic process and biological process this GO :0090280 and positive regulation of calcium ion import and biological process this GO :1900127 and positive regulation of hyaluronan biosynthetic process and biological process this GO :2000379 and positive regulation of reactive oxygen species metabolic process and biological process this GO :2000573 and positive regulation of DNA biosynthetic process and biological process this GO :2000591 and positive regulation of metanephric mesenchymal cell migration and biological process, Extracellular, PDGFB and IDBG-641707 and ENSBTAG00000021697 and 540106, PDGFB and IDBG-8091 and ENSG00000100311 and 5155, Pdgfb and IDBG-163248 and ENSMUSG00000000489 and 18591, c-sis and IBGC5 and PDGF-2 and PDGF2 and SIS and SSV, platelet-derived growth factor binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001568 and blood vessel development and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0002548 and monocyte chemotaxis and biological process this GO :0002576 and platelet degranulation and biological process this GO :0003104 and positive regulation of glomerular filtration and biological process this GO :0005102 and receptor binding and molecular function this GO :0005161 and platelet-derived growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0006260 and DNA replication and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006929 and substrate-dependent cell migration and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007507 and heart development and biological process this GO :0007596 and blood coagulation and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009611 and response to wounding and biological process this GO :0009986 and cell surface and cellular component this GO :0010033 and response to organic substance and biological process this GO :0010512 and negative regulation of phosphatidylinositol biosynthetic process and biological process this GO :0010544 and negative regulation of platelet activation and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014911 and positive regulation of smooth muscle cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0016049 and cell growth and biological process this GO :0016176 and superoxide-generating NADPH oxidase activator activity and molecular function this GO :0016323 and basolateral plasma membrane and cellular component this GO :0018105 and peptidyl-serine phosphorylation and biological process this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0030031 and cell projection assembly and biological process this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030168 and platelet activation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0031954 and positive regulation of protein autophosphorylation and biological process this GO :0032147 and activation of protein kinase activity and biological process this GO :0032148 and activation of protein kinase B activity and biological process this GO :0032355 and response to estradiol and biological process this GO :0032868 and response to insulin and biological process this GO :0035793 and positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway and biological process this GO :0038001 and paracrine signaling and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0042060 and wound healing and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043536 and positive regulation of blood vessel endothelial cell migration and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0043627 and response to estrogen and biological process this GO :0045087 and innate immune response and biological process this GO :0045737 and positive regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045743 and positive regulation of fibroblast growth factor receptor signaling pathway and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045892 and negative regulation of transcription, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005161 : platelet-derived growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0005518 : collagen binding and also this GO :0008083 : growth factor activity and also this GO :0016176 : superoxide-generating NADPH oxidase activator activity and also this GO :0042056 : chemoattractant activity and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity and also this GO :0048407 : platelet-derived growth factor binding, this GO :0005161 : platelet-derived growth factor receptor binding, this GO :0005515 : protein binding, this GO :0005518 : collagen binding, this GO :0008083 : growth factor activity, this GO :0016176 : superoxide-generating NADPH oxidase activator activity, this GO :0042056 : chemoattractant activity, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, this GO :0048407 : platelet-derived growth factor binding, platelet-derived growth factor beta polypeptide |
| Identity: |
8800 |
| Gene: |
PDGFB |
More about : PDGFB |
| Long gene name: |
platelet derived growth factor subunit B |
| Synonyms gene: |
SIS |
| Synonyms gene name: |
platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) platelet-derived growth factor beta polypeptide |
| Synonyms: |
SSV |
| Synonyms name: |
oncogene SIS becaplermin |
| Locus: |
22q13, 1 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
5155 |
| Pubmed identfication: |
2991848 1661670 |
| RefSeq identity: |
NM_002608 |
| Havana BLAST/BLAT: |
OTTHUMG00000151029 |