| Antigen detectable with this ELISA kit: |
Platelet Derived Growth Factor Subunit A (PDGFA) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 12 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA), The concentration of Platelet Derived Growth Factor Subunit A (PDGFA) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Platelet Derived Growth Factor Subunit A (PDGFA), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Platelet Derived Growth Factor Subunit A (PDGFA) |
| Short name: |
Platelet Derived Growth Factor Subunit A (PDGFA) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Platelet Derived Growth Factor functionnal sequence A (platelet-derived growth factor a polypeptide) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, Extracellular, PDGF-A and PDGF1, PDGFA and IDBG-6078 and ENSG00000197461 and 5154, Pdgfa and IDBG-206354 and ENSMUSG00000025856 and 18590, platelet-derived growth factor binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001525 and angiogenesis and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001775 and cell activation and biological process this GO :0001942 and hair follicle development and biological process this GO :0002053 and positive regulation of mesenchymal cell proliferation and biological process this GO :0002576 and platelet degranulation and biological process this GO :0005161 and platelet-derived growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005902 and microvillus and cellular component this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0007596 and blood coagulation and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009611 and response to wounding and biological process this GO :0009790 and embryo development and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0009986 and cell surface and cellular component this GO :0010033 and response to organic substance and biological process this GO :0010035 and response to inorganic substance and biological process this GO :0010512 and negative regulation of phosphatidylinositol biosynthetic process and biological process this GO :0010544 and negative regulation of platelet activation and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0014910 and regulation of smooth muscle cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0030031 and cell projection assembly and biological process this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030168 and platelet activation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0031954 and positive regulation of protein autophosphorylation and biological process this GO :0032355 and response to estradiol and biological process this GO :0032526 and response to retinoic acid and biological process this GO :0032956 and regulation of actin cytoskeleton organization and biological process this GO :0035793 and positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0042493 and response to drug and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043588 and skin development and biological process this GO :0045087 and innate immune response and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048008 and platelet-derived growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048286 and lung alveolus development and biological process this GO :0048407 and platelet-derived growth factor binding and molecular function this GO :0048839 and inner ear development and biological process this GO :0050730 and regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050919 and negative chemotaxis and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0060683 and regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process, this GO :0005161 : platelet-derived growth factor receptor binding, this GO :0005161 : platelet-derived growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0005518 : collagen binding and also this GO :0008083 : growth factor activity and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity and also this GO :0048407 : platelet-derived growth factor binding, this GO :0005515 : protein binding, this GO :0005518 : collagen binding, this GO :0008083 : growth factor activity, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, this GO :0048407 : platelet-derived growth factor binding, 18780 and IDBG-641517 and ENSBTAG00000014541 and 505908, platelet-derived growth factor alpha polypeptide |
| Identity: |
8799 |
| Gene: |
PDGFA |
More about : PDGFA |
| Long gene name: |
platelet derived growth factor subunit A |
| Synonyms gene name: |
platelet-derived growth factor alpha polypeptide |
| Synonyms: |
PDGF1 PDGF-A |
| Synonyms name: |
PDGF A-chain platelet-derived growth factor alpha chain |
| Locus: |
7p22, 3 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
5154 |
| Pubmed identfication: |
1505216 2536956 |
| Havana BLAST/BLAT: |
OTTHUMG00000151412 |