| Antigen detectable with this ELISA kit: |
Pim-1 Oncogene (PIM1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
25ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-1 Oncogene (PIM1), The concentration of Pim-1 Oncogene (PIM1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-1 Oncogene (PIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Pim-1 Oncogene (PIM1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Pim-1 Oncogene (PIM1) |
| Short name: |
Pim-1 Oncogene (PIM1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Pim-1 Oncogene (pim-1 oncogene) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
PIM, PIM1 and IDBG-630304 and ENSBTAG00000000396 and 281402, PIM1 and IDBG-85488 and ENSG00000137193 and 5292, Pim1 and IDBG-160725 and ENSMUSG00000024014 and 18712, alcohol group as acceptor, alcohol group as acceptor and also this GO :0030145 : manganese ion binding and also this GO :0043024 : ribosomal small subunit binding, alcohol group as acceptor and molecular function this GO :0030145 and manganese ion binding and molecular function this GO :0030212 and hyaluronan metabolic process and biological process this GO :0031659 and positive regulation of cyclin-dependent protein kinase activity involved in G1/S and biological process this GO :0043024 and ribosomal small subunit binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0046777 and protein autophosphorylation and biological process, nuclei, this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007049 and cell cycle and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0009103 and lipopolysaccharide biosynthetic process and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008134 : transcription factor binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008134 : transcription factor binding, this GO :0016772 : transferase activity, this GO :0016773 : phosphotransferase activity, this GO :0030145 : manganese ion binding, this GO :0043024 : ribosomal small subunit binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0016773 : phosphotransferase activity, transferring phosphorus-containing groups and molecular function this GO :0016773 and phosphotransferase activity, pim-1 oncogene |
| Identity: |
8986 |
| Gene: |
PIM1 |
More about : PIM1 |
| Long gene name: |
Pim-1 proto-oncogene, serine/threonine kinase |
| Synonyms gene: |
PIM |
| Synonyms gene name: |
pim-1 oncogene |
| Locus: |
6p21, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
5292 |
| Havana BLAST/BLAT: |
OTTHUMG00000016426 |