| Antigen detectable with this ELISA kit: |
Phospholipase D2 (PLD2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
55ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase D2 (PLD2), The concentration of Phospholipase D2 (PLD2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D2 (PLD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Phospholipase D2 (PLD2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Phospholipase D2 (PLD2) |
| Short name: |
Phospholipase D2 (PLD2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Phospholipase D2 (phospholipase D2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
N-acylphosphatidylethanolamine-specific phospholipase D activity, PLD2 and IDBG-19793 and ENSG00000129219 and 5338, PLD2 and IDBG-634274 and ENSBTAG00000013392 and 522159, Plasma membranes, Pld2 and IDBG-194655 and ENSMUSG00000020828 and 18806, this GO :0002031 and G-protein coupled receptor internalization and biological process this GO :0003824 and catalytic activity and molecular function this GO :0004630 and phospholipase D activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006644 and phospholipid metabolic process and biological process this GO :0006654 and phosphatidic acid biosynthetic process and biological process this GO :0006655 and phosphatidylglycerol biosynthetic process and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0007010 and cytoskeleton organization and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0008152 and metabolic process and biological process this GO :0016042 and lipid catabolic process and biological process this GO :0031526 and brush border membrane and cellular component this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0046474 and glycerophospholipid biosynthetic process and biological process this GO :0070290 and N-acylphosphatidylethanolamine-specific phospholipase D activity and molecular function, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004630 : phospholipase D activity and also this GO :0005515 : protein binding and also this GO :0035091 : phosphatidylinositol binding and also this GO :0070290 : N-acylphosphatidylethanolamine-specific phospholipase D activity, this GO :0004630 : phospholipase D activity, this GO :0005515 : protein binding, this GO :0035091 : phosphatidylinositol binding, this GO :0070290 : N-acylphosphatidylethanolamine-specific phospholipase D activity, phospholipase D2 |
| Identity: |
9068 |
| Gene: |
PLD2 |
More about : PLD2 |
| Long gene name: |
phospholipase D2 |
| Synonyms name: |
choline phosphatase 2 |
| Locus: |
17p13, 2 |
| Discovery year: |
1998-08-21 |
| GenBank acession: |
AF035483 |
| Entrez gene record: |
5338 |
| Pubmed identfication: |
9858823 9582313 |
| RefSeq identity: |
NM_002663 |
| Classification: |
Phospholipases |
| Havana BLAST/BLAT: |
OTTHUMG00000090779 |