| Antigen detectable with this ELISA kit: |
Group V (PLA2G5), Phospholipase A2 |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 37pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Group V (PLA2G5), Group V (PLA2G5), Group V (PLA2G5), Group V (PLA2G5) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, The concentration of Phospholipase A2, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Phospholipase A2, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Group V (PLA2G5), Phospholipase A2 |
| Short name: |
Group V (PLA2G5) ELISA kit, Phospholipase A2 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
family V (phospholipase A2, family V) Enzyme-linked immunosorbent assay reagent, Phospholipase A2 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, Extracellular, FRFB and GV-PLA2 and hVPLA(2) and PLA2-10, PLA2G5 and IDBG-92814 and ENSG00000127472 and 5322, Pla2g5 and IDBG-200004 and ENSMUSG00000041193 and 18784, calcium-dependent phospholipase A2 activity, group V, this GO :0005509 and calcium ion binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006644 and phospholipid metabolic process and biological process this GO :0006654 and phosphatidic acid biosynthetic process and biological process this GO :0006663 and platelet activating factor biosynthetic process and biological process this GO :0008201 and heparin binding and molecular function this GO :0009986 and cell surface and cellular component this GO :0016042 and lipid catabolic process and biological process this GO :0019370 and leukotriene biosynthetic process and biological process this GO :0034097 and response to cytokine and biological process this GO :0036148 and phosphatidylglycerol acyl-chain remodeling and biological process this GO :0036149 and phosphatidylinositol acyl-chain remodeling and biological process this GO :0036150 and phosphatidylserine acyl-chain remodeling and biological process this GO :0036151 and phosphatidylcholine acyl-chain remodeling and biological process this GO :0036152 and phosphatidylethanolamine acyl-chain remodeling and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046474 and glycerophospholipid biosynthetic process and biological process this GO :0047498 and calcium-dependent phospholipase A2 activity and molecular function this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050482 and arachidonic acid secretion and biological process this GO :0051591 and response to cAMP and biological process, this GO :0005509 : calcium ion binding, this GO :0005509 : calcium ion binding and also this GO :0008201 : heparin binding and also this GO :0047498 : calcium-dependent phospholipase A2 activity, this GO :0008201 : heparin binding, this GO :0047498 : calcium-dependent phospholipase A2 activity, 84824 and IDBG-645684 and ENSBTAG00000039122 and 614911, phospholipase A2 |
| Identity: |
9038 |
| Gene: |
PLA2G5 |
More about : PLA2G5 |
| Long gene name: |
phospholipase A2 group V |
| Synonyms gene name: |
group V , phospholipase A2 |
| Locus: |
1p36, 13 |
| Discovery year: |
1994-11-30 |
| GenBank acession: |
U03090 |
| Entrez gene record: |
5322 |
| Pubmed identfication: |
8838795 8300559 |
| RefSeq identity: |
NM_000929 |
| Classification: |
Phospholipases |
| Havana BLAST/BLAT: |
OTTHUMG00000002698 |