| Antigen detectable with this ELISA kit: |
Group IIA (PLA2G2A), Phospholipase A2 |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
116ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Group IIA (PLA2G2A), Group IIA (PLA2G2A), Group IIA (PLA2G2A), Group IIA (PLA2G2A) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, The concentration of Phospholipase A2, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Phospholipase A2, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Group IIA (PLA2G2A), Phospholipase A2 |
| Short name: |
Group IIA (PLA2G2A) ELISA kit, Phospholipase A2 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
family IIA (phospholipase A2, family IIA (platelets, synovial fluid)) Enzyme-linked immunosorbent assay reagent, Phospholipase A2 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, MOM1 and PLA2 and PLA2B and PLA2L and PLA2S and PLAS1 and sPLA2, PLA2G2A and IDBG-92794 and ENSG00000188257 and 5320, Pla2g2a and IDBG-200035 and ENSMUSG00000058908 and 18780, calcium-dependent phospholipase A2 activity, group IIA (platelets, synovial fluid), this GO :0004623 and phospholipase A2 activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006644 and phospholipid metabolic process and biological process this GO :0006654 and phosphatidic acid biosynthetic process and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0016042 and lipid catabolic process and biological process this GO :0030141 and secretory granule and cellular component this GO :0034374 and low-density lipoprotein particle remodeling and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0036148 and phosphatidylglycerol acyl-chain remodeling and biological process this GO :0036149 and phosphatidylinositol acyl-chain remodeling and biological process this GO :0036150 and phosphatidylserine acyl-chain remodeling and biological process this GO :0036151 and phosphatidylcholine acyl-chain remodeling and biological process this GO :0036152 and phosphatidylethanolamine acyl-chain remodeling and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046473 and phosphatidic acid metabolic process and biological process this GO :0046474 and glycerophospholipid biosynthetic process and biological process this GO :0047498 and calcium-dependent phospholipase A2 activity and molecular function this GO :0050678 and regulation of epithelial cell proliferation and biological process this GO :0050680 and negative regulation of epithelial cell proliferation and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process, this GO :0004623 : phospholipase A2 activity, this GO :0004623 : phospholipase A2 activity and also this GO :0005509 : calcium ion binding and also this GO :0005543 : phospholipid binding and also this GO :0047498 : calcium-dependent phospholipase A2 activity, this GO :0005509 : calcium ion binding, this GO :0005543 : phospholipid binding, this GO :0047498 : calcium-dependent phospholipase A2 activity, phospholipase A2 |
| Identity: |
9031 |
| Gene: |
PLA2G2A |
More about : PLA2G2A |
| Long gene name: |
phospholipase A2 group IIA |
| Synonyms gene: |
PLA2B PLA2L |
| Synonyms gene name: |
group IIA (platelets, phospholipase A2, synovial fluid) |
| Locus: |
1p36, 13 |
| Discovery year: |
1989-04-24 |
| GenBank acession: |
BC005919 |
| Entrez gene record: |
5320 |
| Pubmed identfication: |
8838795 |
| RefSeq identity: |
NM_000300 |
| Classification: |
Phospholipases |
| Havana BLAST/BLAT: |
OTTHUMG00000002699 |