| Antigen detectable with this ELISA kit: |
Brain (PGAM1), Phosphoglycerate Mutase 1 |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
55ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Brain (PGAM1), Brain (PGAM1), Brain (PGAM1), Brain (PGAM1) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoglycerate Mutase 1, The concentration of Phosphoglycerate Mutase 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate Mutase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Phosphoglycerate Mutase 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Brain (PGAM1), Phosphoglycerate Mutase 1 |
| Short name: |
Brain (PGAM1) ELISA kit, Phosphoglycerate Mutase 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Brain (phosphoglycerate mutase 1 (brain)) Enzyme-linked immunosorbent assay reagent, Phosphoglycerate Mutase 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
HEL-S-35 and PGAM-B and PGAMA, PGAM1 and IDBG-84719 and ENSG00000171314 and 441531, Plasma membranes, intramolecular transferase activity, phosphotransferases, phosphotransferases and also this GO :0019901 : protein kinase binding, phosphotransferases and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0043456 and regulation of pentose-phosphate shunt and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045730 and respiratory burst and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004082 and bisphosphoglycerate mutase activity and molecular function this GO :0004083 and bisphosphoglycerate 2-phosphatase activity and molecular function this GO :0004619 and phosphoglycerate mutase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006094 and gluconeogenesis and biological process this GO :0006096 and glycolytic process and biological process this GO :0006110 and regulation of glycolytic process and biological process this GO :0016020 and membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016868 and intramolecular transferase activity, this GO :0004082 : bisphosphoglycerate mutase activity, this GO :0004082 : bisphosphoglycerate mutase activity and also this GO :0004083 : bisphosphoglycerate 2-phosphatase activity and also this GO :0004619 : phosphoglycerate mutase activity and also this GO :0005515 : protein binding and also this GO :0016868 : intramolecular transferase activity, this GO :0004083 : bisphosphoglycerate 2-phosphatase activity, this GO :0004619 : phosphoglycerate mutase activity, this GO :0005515 : protein binding, this GO :0016868 : intramolecular transferase activity, this GO :0019901 : protein kinase binding, 5223, phosphoglycerate mutase 1 (brain) |
| Identity: |
8888 |
| Gene: |
PGAM1 |
More about : PGAM1 |
| Long gene name: |
phosphoglycerate mutase 1 |
| Synonyms gene: |
PGAMA |
| Synonyms gene name: |
phosphoglycerate mutase 1 (brain) |
| Synonyms: |
PGAM-B |
| Synonyms name: |
Phosphoglycerate mutase A, nonmuscle form |
| Locus: |
10q24, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
BC010038 |
| Entrez gene record: |
5223 |
| Pubmed identfication: |
2846553 |
| RefSeq identity: |
NM_002629 |
| Classification: |
Bisphosphoglycerate phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000018846 |