| Antigen detectable with this ELISA kit: |
cGMP Inhibited (PDE3A), Phosphodiesterase 3A |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
27ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 3A, The concentration of Phosphodiesterase 3A, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, cGMP Inhibited (PDE3A), cGMP Inhibited (PDE3A), cGMP Inhibited (PDE3A), cGMP Inhibited (PDE3A) in the samples is then determined by comparing the O, of the samples to the standard curve, only those wells that contain Phosphodiesterase 3A, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
cGMP Inhibited (PDE3A), Phosphodiesterase 3A |
| Short name: |
cGMP Inhibited (PDE3A) ELISA kit, Phosphodiesterase 3A |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
cGMP Inhibited (phosphodiesterase 3A, cGMP-inhibited) Enzyme-linked immunosorbent assay reagent, Phosphodiesterase 3A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
3', CGI-PDE and CGI-PDE A and CGI-PDE-A, PDE3A and IDBG-22315 and ENSG00000172572 and 5139, PDE3A and IDBG-639031 and ENSBTAG00000017260 and 536636, Pde3a and IDBG-196288 and ENSMUSG00000041741 and 54611, Plasma membranes, cGMP-inhibited, this GO :0001556 and oocyte maturation and biological process this GO :0004114 and 3', this GO :0004114 : 3', this GO :0004114 : 3', this GO :0004115 : 3', this GO :0004119 : cGMP-inhibited cyclic-nucleotide phosphodiesterase activity, this GO :0030552 : cAMP binding, this GO :0046872 : metal ion binding, 5'-cyclic-AMP phosphodiesterase activity, 5'-cyclic-AMP phosphodiesterase activity and also this GO :0004119 : cGMP-inhibited cyclic-nucleotide phosphodiesterase activity and also this GO :0030552 : cAMP binding and also this GO :0046872 : metal ion binding, 5'-cyclic-AMP phosphodiesterase activity and molecular function this GO :0004119 and cGMP-inhibited cyclic-nucleotide phosphodiesterase activity and molecular function this GO :0005829 and cytosol and cellular component this GO :0006198 and cAMP catabolic process and biological process this GO :0006629 and lipid metabolic process and biological process this GO :0007165 and signal transduction and biological process this GO :0007596 and blood coagulation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016101 and diterpenoid metabolic process and biological process this GO :0019933 and cAMP-mediated signaling and biological process this GO :0019934 and cGMP-mediated signaling and biological process this GO :0030552 and cAMP binding and molecular function this GO :0040020 and regulation of meiosis and biological process this GO :0042493 and response to drug and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043116 and negative regulation of vascular permeability and biological process this GO :0043117 and positive regulation of vascular permeability and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051591 and response to cAMP and biological process this GO :0060282 and positive regulation of oocyte development and biological process this GO :0071321 and cellular response to cGMP and biological process this GO :0071560 and cellular response to transforming growth factor beta stimulus and biological process, 5'-cyclic-nucleotide phosphodiesterase activity, 5'-cyclic-nucleotide phosphodiesterase activity and also this GO :0004115 : 3', 5'-cyclic-nucleotide phosphodiesterase activity and molecular function this GO :0004115 and 3', phosphodiesterase 3A |
| Identity: |
8778 |
| Gene: |
PDE3A |
More about : PDE3A |
| Long gene name: |
phosphodiesterase 3A |
| Synonyms gene name: |
cGMP-inhibited , phosphodiesterase 3A |
| Synonyms: |
CGI-PDE |
| Locus: |
12p12, 2 |
| Discovery year: |
1994-07-29 |
| Entrez gene record: |
5139 |
| Pubmed identfication: |
1315035 10679291 |
| Classification: |
Phosphodiesterases |
| Havana BLAST/BLAT: |
OTTHUMG00000168962 |