| Antigen detectable with this ELISA kit: |
O-6-Methylguanine DNA Methyltransferase (MGMT) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT), The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
O-6-Methylguanine DNA Methyltransferase (MGMT) |
| Short name: |
O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
O-6-Methylguanine Desoxyribonucleic acid Methyltransferase (O-6-methylguanine-Desoxyribonucleic acid methyltransferase) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-methyltransferase activity, MGMT and IDBG-93188 and ENSG00000170430 and 4255, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314, nuclei, this GO :0003677 and DNA binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0003908 and methylated-DNA-[protein]-cysteine S-methyltransferase activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006266 and DNA ligation and biological process this GO :0006281 and DNA repair and biological process this GO :0006306 and DNA methylation and biological process this GO :0006307 and DNA dealkylation involved in DNA repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008168 and methyltransferase activity and molecular function this GO :0009008 and DNA-methyltransferase activity and molecular function this GO :0009636 and response to toxic substance and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0034599 and cellular response to oxidative stress and biological process this GO :0042493 and response to drug and biological process this GO :0043281 and regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045739 and positive regulation of DNA repair and biological process this GO :0051593 and response to folic acid and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071479 and cellular response to ionizing radiation and biological process, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003824 : catalytic activity and also this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity and also this GO :0005509 : calcium ion binding and also this GO :0008168 : methyltransferase activity and also this GO :0009008 : DNA-methyltransferase activity, this GO :0003824 : catalytic activity, this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity, this GO :0005509 : calcium ion binding, this GO :0008168 : methyltransferase activity, this GO :0009008 : DNA-methyltransferase activity, O-6-methylguanine-DNA methyltransferase |
| Identity: |
7059 |
| Gene: |
MGMT |
More about : MGMT |
| Long gene name: |
O-6-methylguanine-DNA methyltransferase |
| Synonyms name: |
methylated-DNA--protein-cysteine methyltransferase |
| Locus: |
10q26, 3 |
| Discovery year: |
1989-10-16 |
| GenBank acession: |
M29971 |
| Entrez gene record: |
4255 |
| RefSeq identity: |
NM_002412 |
| Havana BLAST/BLAT: |
OTTHUMG00000019261 |