| Antigen detectable with this ELISA kit: |
Cytoplasmic 2 (NFATC2), Nuclear Factor Of Activated T-Cells |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Cytoplasmic 2 (NFATC2), Cytoplasmic 2 (NFATC2), Cytoplasmic 2 (NFATC2), Cytoplasmic 2 (NFATC2) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Of Activated T-Cells, The concentration of Nuclear Factor Of Activated T-Cells, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Of Activated T-Cells, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Nuclear Factor Of Activated T-Cells, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cytoplasmic 2 (NFATC2), Factor Of Activated T-Cells |
| Short name: |
Cytoplasmic 2 (NFATC2) ELISA kit, Nuclear Factor Of Activated T-Cells |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cytoplasmic 2 (nuclear factor on activated T-cells, calcineurin-dependent 2) Enzyme-linked immunosorbent assay reagent, cytoplasmic, Nuclear Factor on Activated T-Cells |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0015629 and actin cytoskeleton and cellular component this GO :0016477 and cell migration and biological process this GO :0030529 and ribonucleoprotein complex and cellular component this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042493 and response to drug and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0044798 and nuclear transcription factor complex and cellular component this GO :0045087 and innate immune response and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050853 and B cell receptor signaling pathway and biological process, NFAT1 and NFATP, NFATC2 and IDBG-643253 and ENSBTAG00000018270 and 530401, NFATC2 and IDBG-81223 and ENSG00000101096 and 4773, Nfatc2 and IDBG-213130 and ENSMUSG00000027544 and 18019, calcineurin-dependent 2, cytoplasmic, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001816 and cytokine production and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, transcription regulatory region DNA binding, nuclear factor of activated T-cells |
| Identity: |
7776 |
| Gene: |
NFATC2 |
More about : NFATC2 |
| Long gene name: |
nuclear factor of activated T-cells 2 |
| Synonyms gene name: |
calcineurin-dependent 2 , cytoplasmic, nuclear factor of activated T-cells |
| Synonyms: |
NF-ATP NFATp NFAT1 |
| Locus: |
20q13, 2 |
| Discovery year: |
1994-11-16 |
| GenBank acession: |
U43342 U43341 |
| Entrez gene record: |
4773 |
| Pubmed identfication: |
8202141 |
| RefSeq identity: |
NM_012340 |
| Classification: |
Nuclear factors of activated T-cells |
| Havana BLAST/BLAT: |
OTTHUMG00000032747 |