| Antigen detectable with this ELISA kit: |
Light Polypeptide (NEFL), Neurofilament |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cerebrospinal fluid and other biological fluids, plasma, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Light Polypeptide (NEFL), Light Polypeptide (NEFL), Light Polypeptide (NEFL), Light Polypeptide (NEFL) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, The concentration of Neurofilament, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Neurofilament, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Light Polypeptide (NEFL), Neurofilament |
| Short name: |
Light Polypeptide (NEFL) ELISA kit, Neurofilament |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Light multipeptide (neurofilament, light polypeptide) Enzyme-linked immunosorbent assay reagent, Neurofilament |
| Alternative technique: |
peptides |
| Alternative to gene target: |
CMT1F and CMT2E and NF-L and NF68 and NFL and PPP1R110, Cytoplasm, NEFL and IDBG-635366 and ENSBTAG00000021949 and 281348, NEFL and IDBG-777808 and ENSG00000277586 and 4747, Nefl and IDBG-176493 and ENSMUSG00000022055 and 18039, bridging, bridging and also this GO :0042802 : identical protein binding and also this GO :0043274 : phospholipase binding, bridging and molecular function this GO :0031133 and regulation of axon diameter and biological process this GO :0033596 and TSC1-TSC2 complex and cellular component this GO :0033693 and neurofilament bundle assembly and biological process this GO :0040011 and locomotion and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0043274 and phospholipase binding and molecular function this GO :0043434 and response to peptide hormone and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045109 and intermediate filament organization and biological process this GO :0045110 and intermediate filament bundle assembly and biological process this GO :0048812 and neuron projection morphogenesis and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0050885 and neuromuscular process controlling balance and biological process this GO :0051258 and protein polymerization and biological process this GO :0051412 and response to corticosterone and biological process this GO :0060052 and neurofilament cytoskeleton organization and biological process, light polypeptide, protein binding, this GO :0000226 and microtubule cytoskeleton organization and biological process this GO :0005198 and structural molecule activity and molecular function this GO :0005200 and structural constituent of cytoskeleton and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005882 and intermediate filament and cellular component this GO :0005883 and neurofilament and cellular component this GO :0007268 and synaptic transmission and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008089 and anterograde axon car this GO transport and biological process this GO :0008090 and retrograde axon car this GO transport and biological process this GO :0008219 and cell death and biological process this GO :0009636 and response to toxic substance and biological process this GO :0010033 and response to organic substance and biological process this GO :0010243 and response to organonitrogen compound and biological process this GO :0014012 and peripheral nervous system axon regeneration and biological process this GO :0019896 and axon transport of mitochondrion and biological process this GO :0019904 and protein domain specific binding and molecular function this GO :0030424 and axon and cellular component this GO :0030426 and growth cone and cellular component this GO :0030674 and protein binding, this GO :0005198 : structural molecule activity, this GO :0005198 : structural molecule activity and also this GO :0005200 : structural constituent of cytoskeleton and also this GO :0005515 : protein binding and also this GO :0008022 : protein C-terminus binding and also this GO :0019904 : protein domain specific binding and also this GO :0030674 : protein binding, this GO :0005200 : structural constituent of cytoskeleton, this GO :0005515 : protein binding, this GO :0008022 : protein C-terminus binding, this GO :0019904 : protein domain specific binding, this GO :0030674 : protein binding, this GO :0042802 : identical protein binding, this GO :0043274 : phospholipase binding, neurofilament |
| Identity: |
7739 |
| Gene: |
NEFL |
More about : NEFL |
| Long gene name: |
neurofilament light |
| Synonyms gene name: |
light polypeptide , light polypeptide 68kDa neurofilament, neurofilament |
| Synonyms: |
NFL CMT1F CMT2E NF68 PPP1R110 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 110 |
| Locus: |
8p21, 2 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
4747 |
| Pubmed identfication: |
17620486 3145240 |
| RefSeq identity: |
NM_006158 |
| Classification: |
Intermediate filaments Type IV Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000134284 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_259 |