| Antigen detectable with this ELISA kit: |
Nerve Growth Factor (NGF) |
| Species reactivity: |
Porcine (Pig), Sus scrofa |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor (NGF), The concentration of Nerve Growth Factor (NGF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor (NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Nerve Growth Factor (NGF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Nerve Growth Factor (NGF) |
| Short name: |
Nerve Growth Factor (NGF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Nerve Growth Factor (nerve growth factor (beta polypeptide)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Beta-NGF and HSAN5 and NGFB, Extracellular, NGF and IDBG-101347 and ENSG00000134259 and 4803, NGF and IDBG-648895 and ENSBTAG00000007446 and 281350, Ngf and IDBG-180362 and ENSMUSG00000027859 and 18049, growth factor activity, this GO :0000186 and activation of MAPKK activity and biological process this GO :0005057 and receptor signaling protein activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005163 and nerve growth factor receptor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005768 and endosome and cellular component this GO :0005796 and this GO lgi lumen and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007202 and activation of phospholipase C activity and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007422 and peripheral nervous system development and biological process this GO :0007613 and memory and biological process this GO :0007623 and circadian rhythm and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008344 and adult locomotory behavior and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0009314 and response to radiation and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0010033 and response to organic substance and biological process this GO :0010193 and response to ozone and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010976 and positive regulation of neuron projection development and biological process this GO :0014042 and positive regulation of neuron maturation and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0019233 and sensory perception of pain and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0031175 and neuron projection development and biological process this GO :0031954 and positive regulation of protein autophosphorylation and biological process this GO :0032455 and nerve growth factor processing and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0035094 and response to nicotine and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042493 and response to drug and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043281 and regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045664 and regulation of neuron differentiation and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045773 and positive regulation of axon extension and biological process this GO :0045786 and negative regulation of cell cycle and biological process this GO :0046928 and regulation of neurotransmitter secretion and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048812 and neuron projection morphogenesis and biological process this GO :0050770 and regulation of axonogenesis and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051388 and positive regulation of nerve growth factor receptor signaling pathway and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :0051602 and response to electrical stimulus and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :2000648 and positive regulation of stem cell proliferation and biological process this GO :2000675 and negative regulation of type B pancreatic cell apoptotic process and biological process, this GO :0005057 : receptor signaling protein activity, this GO :0005057 : receptor signaling protein activity and also this GO :0005102 : receptor binding and also this GO :0005163 : nerve growth factor receptor binding and also this GO :0008083 : growth factor activity, this GO :0005102 : receptor binding, this GO :0005163 : nerve growth factor receptor binding, this GO :0008083 : growth factor activity, nerve growth factor (beta polypeptide) |
| Identity: |
7808 |
| Gene: |
NGF |
More about : NGF |
| Long gene name: |
nerve growth factor |
| Synonyms gene: |
NGFB |
| Synonyms gene name: |
nerve growth factor (beta polypeptide) |
| Locus: |
1p13, 2 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
4803 |
| RefSeq identity: |
NM_002506 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000011880 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_260 |