| Antigen detectable with this ELISA kit: |
Nephronectin (NPNT) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nephronectin (NPNT), The concentration of Nephronectin (NPNT) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nephronectin (NPNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Nephronectin (NPNT), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Nephronectin (NPNT) |
| Short name: |
Nephronectin (NPNT) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Nephronectin (nephronectin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, NPNT and IDBG-33102 and ENSG00000168743 and 255743, NPNT and IDBG-640632 and ENSBTAG00000006686 and 513362, Npnt and IDBG-194680 and ENSMUSG00000040998 and 114249, protein binding, this GO :0001657 and ureteric bud development and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0005178 and integrin binding and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005604 and basement membrane and cellular component this GO :0007160 and cell-matrix adhesion and biological process this GO :0010694 and positive regulation of alkaline phosphatase activity and biological process this GO :0010811 and positive regulation of cell-substrate adhesion and biological process this GO :0016020 and membrane and cellular component this GO :0030198 and extracellular matrix organization and biological process this GO :0030485 and smooth muscle contractile fiber and cellular component this GO :0030511 and positive regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0033631 and cell-cell adhesion mediated by integrin and biological process this GO :0034678 and alpha8-beta1 integrin complex and cellular component this GO :0045184 and establishment of protein localization and biological process this GO :0045669 and positive regulation of osteoblast differentiation and biological process this GO :0045987 and positive regulation of smooth muscle contraction and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0097195 and pilomotor reflex and biological process this GO :2000721 and positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation and biological process, this GO :0005178 : integrin binding, this GO :0005178 : integrin binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, nephronectin |
| Identity: |
27405 |
| Gene: |
NPNT |
More about : NPNT |
| Long gene name: |
nephronectin |
| Synonyms: |
EGFL6L POEM |
| Locus: |
4q24 |
| Discovery year: |
2005-10-07 |
| Entrez gene record: |
255743 |
| Pubmed identfication: |
15754038 |
| RefSeq identity: |
NM_198278 |
| Havana BLAST/BLAT: |
OTTHUMG00000161189 |