| Antigen detectable with this ELISA kit: |
Necdin (NDN) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
066ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Necdin (NDN), The concentration of Necdin (NDN) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Necdin (NDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Necdin (NDN), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Necdin (NDN) |
| Short name: |
Necdin (NDN) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Necdin (necdin homolog (mouse)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0007399 and nervous system development and biological process this GO :0007409 and axonogenesis and biological process this GO :0007413 and axonal fasciculation and biological process this GO :0007417 and central nervous system development and biological process this GO :0007585 and respiratory gaseous exchange and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008347 and glial cell migration and biological process this GO :0009791 and post-embryonic development and biological process this GO :0019233 and sensory perception of pain and biological process this GO :0040008 and regulation of growth and biological process this GO :0042995 and cell projection and cellular component this GO :0043015 and gamma-tubulin binding and molecular function this GO :0043204 and perikaryon and cellular component this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048666 and neuron development and biological process this GO :0048675 and axon extension and biological process this GO :0048871 and multicellular organismal homeostasis and biological process this GO :0071514 and genetic imprinting and biological process, HsT16328 and PWCR, NDN and IDBG-4155 and ENSG00000182636 and 4692, NDN and IDBG-647846 and ENSBTAG00000002186 and 386664, Ndn and IDBG-190587 and ENSMUSG00000033585 and 17984, gamma-tubulin binding, nuclei, this GO :0001764 and neuron migration and biological process this GO :0003016 and respiratory system process and biological process this GO :0003677 and DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0005515 : protein binding and also this GO :0043015 : gamma-tubulin binding, this GO :0005515 : protein binding, this GO :0043015 : gamma-tubulin binding, necdin homolog (mouse) |
| Identity: |
7675 |
| Gene: |
NDN |
More about : NDN |
| Long gene name: |
MAGE family member , necdin |
| Synonyms gene name: |
melanoma antigen (MAGE) family member , necdin homolog (mouse) necdin |
| Synonyms: |
HsT16328 PWCR |
| Synonyms name: |
Prader-Willi syndrome chromosome region |
| Locus: |
15q11, 2 |
| Discovery year: |
1997-10-13 |
| GenBank acession: |
U35139 |
| Entrez gene record: |
4692 |
| Pubmed identfication: |
9302265 |
| RefSeq identity: |
NM_002487 |
| Classification: |
MAGE family |
| Havana BLAST/BLAT: |
OTTHUMG00000129161 |
| Locus Specific Databases: |
LRG_1047 |