| Antigen detectable with this ELISA kit: |
Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
056ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), The concentration of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) |
| Short name: |
Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Mitogen Activated Protein phosphorylation catalyst Kinase 1 (mitogen-activated protein phosphorylation catalyst kinase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CFC3 and MAPKK1 and MEK1 and MKK1 and PRKMK1, MAP2K1 and IDBG-18019 and ENSG00000169032 and 5604, MAP2K1 and IDBG-645446 and ENSBTAG00000033983 and 533199, Map2k1 and IDBG-176099 and ENSMUSG00000004936 and 26395, nuclei, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0000187 and activation of MAPK activity and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0003056 and regulation of vascular smooth muscle contraction and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004708 and MAP kinase kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004728 and receptor signaling protein tyrosine phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005769 and early endosome and cellular component this GO :0005770 and late endosome and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005815 and microtubule organizing center and cellular component this GO :0005829 and cytosol and cellular component this GO :0005874 and microtubule and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0005938 and cell cortex and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006928 and cellular component movement and biological process this GO :0006935 and chemotaxis and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007067 and mitotic nuclear division and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004708 : MAP kinase kinase activity and also this GO :0004712 : protein serine/threonine/tyrosine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004728 : receptor signaling protein tyrosine phosphatase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004708 : MAP kinase kinase activity, this GO :0004712 : protein serine/threonine/tyrosine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004728 : receptor signaling protein tyrosine phosphatase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0017016 : Ras GTPase binding, this GO :0019901 : protein kinase binding, this GO :0031435 : mitogen-activated protein kinase kinase kinase binding, this GO :0043539 : protein serine/threonine kinase activator activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0017016 : Ras GTPase binding and also this GO :0019901 : protein kinase binding and also this GO :0031435 : mitogen-activated protein kinase kinase kinase binding and also this GO :0043539 : protein serine/threonine kinase activator activity, transferring phosphorus-containing groups and molecular function this GO :0017016 and Ras GTPase binding and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030182 and neuron differentiation and biological process this GO :0030216 and keratinocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0031435 and mitogen-activated protein kinase kinase kinase binding and molecular function this GO :0032320 and positive regulation of Ras GTPase activity and biological process this GO :0032402 and melanosome transport and biological process this GO :0032839 and dendrite cytoplasm and cellular component this GO :0032872 and regulation of stress-activated MAPK cascade and biological process this GO :0032968 and positive regulation of transcription elongation from RNA polymerase II promoter and biological process this GO :0034111 and negative regulation of homotypic cell-cell adhesion and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0043204 and perikaryon and cellular component this GO :0043539 and protein serine/threonine kinase activator activity and molecular function this GO :0045087 and innate immune response and biological process this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0046579 and positive regulation of Ras protein signal transduction and biological process this GO :0047496 and vesicle transport along microtubule and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048313 and this GO lgi inheritance and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048678 and response to axon injury and biological process this GO :0048812 and neuron projection morphogenesis and biological process this GO :0048870 and cell motility and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051403 and stress-activated MAPK cascade and biological process this GO :0060674 and placenta blood vessel development and biological process this GO :0060711 and labyrinthine layer development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071902 and positive regulation of protein serine/threonine kinase activity and biological process this GO :0090170 and regulation of this GO lgi inheritance and biological process this GO :0090398 and cellular senescence and biological process this GO :2000641 and regulation of early endosome to late endosome transport and biological process, mitogen-activated protein kinase kinase 1 |
| Identity: |
6840 |
| Gene: |
MAP2K1 |
More about : MAP2K1 |
| Long gene name: |
mitogen-activated protein kinase kinase 1 |
| Synonyms gene: |
PRKMK1 |
| Synonyms: |
MEK1 MAPKK1 |
| Locus: |
15q22, 31 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
L11284 |
| Entrez gene record: |
5604 |
| Pubmed identfication: |
9465908 8388392 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000133196 |
| Locus Specific Databases: |
The Globin Gene Server LRG_725 |