| Antigen detectable with this ELISA kit: |
Microphthalmia Associated Transcription Factor (MITF) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
053ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microphthalmia Associated Transcription Factor (MITF), The concentration of Microphthalmia Associated Transcription Factor (MITF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microphthalmia Associated Transcription Factor (MITF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Microphthalmia Associated Transcription Factor (MITF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Microphthalmia Associated Transcription Factor (MITF) |
| Short name: |
Microphthalmia Associated Transcription Factor (MITF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Microphthalmia Associated Transcription Factor (microphthalmia-associated transcription factor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006461 and protein complex assembly and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0030154 and cell differentiation and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043010 and camera-type eye development and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0043473 and pigmentation and biological process this GO :0044336 and canonical Wnt signaling pathway involved in negative regulation of apoptotic process and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045670 and regulation of osteoclast differentiation and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046849 and bone remodeling and biological process this GO :0046983 and protein dimerization activity and molecular function, MITF and IDBG-44086 and ENSG00000187098 and 4286, Mitf and IDBG-172461 and ENSMUSG00000035158 and 17342, bHLHe32 and CMM8 and MI and WS2 and WS2A, nuclei, protein dimerization activity, this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006355 and regulation of transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0046983 : protein dimerization activity, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0046983 : protein dimerization activity, 29882 and IDBG-644985 and ENSBTAG00000006679 and 407219, microphthalmia-associated transcription factor |
| Identity: |
7105 |
| Gene: |
MITF |
More about : MITF |
| Long gene name: |
melanogenesis associated transcription factor |
| Synonyms gene: |
WS2A WS2 |
| Synonyms gene name: |
Waardenburg syndrome, type 2A microphthalmia-associated transcription factor |
| Synonyms: |
MI bHLHe32 |
| Synonyms name: |
homolog of mouse microphthalmia |
| Locus: |
3p13 |
| Discovery year: |
1993-10-27 |
| Entrez gene record: |
4286 |
| Pubmed identfication: |
8069297 7874167 7951321 |
| RefSeq identity: |
NM_198159 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000149921 |
| Locus Specific Databases: |
LOVD - Leiden Open Variation Database LRG_776 |