| Antigen detectable with this ELISA kit: |
Melanoma Cell Adhesion Molecule (MCAM) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
100ng/mL, 12, 25ng/mL, 3, 50ng/mL, 6, 125-200ng/mL The standard curve concentrations used for the ELISA&rsquo, 125ng/mL, 25ng/mL, 3, 5ng/mL, s were 200ng/mL |
| Sensitivity: |
44ng/mL, The minimum detectable dose of this kit is typically less than 1 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM), The concentration of Melanoma Cell Adhesion Molecule (MCAM) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Melanoma Cell Adhesion Molecule (MCAM), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
present in lysates used as reference for ELISA quantification of these molecules and their subunits, Whole adhesion and interacting molecules are |
| Gene target: |
Melanoma Adhesion Molecule (MCAM) |
| Short name: |
Melanoma Cell Adhesion Molecule (MCAM) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Melanoma cellular Adhesion Molecule (melanoma cell adhesion molecule) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD146 and MUC18, MCAM and IDBG-631776 and ENSBTAG00000006835 and 281301, MCAM and IDBG-74428 and ENSG00000076706 and 102465453, Mcam and IDBG-155835 and ENSMUSG00000032135 and 84004, nuclei, protein binding, this GO :0003094 and glomerular filtration and biological process this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030335 and positive regulation of cell migration and biological process this GO :0061042 and vascular wound healing and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, 4162, melanoma cell adhesion molecule |
| Identity: |
6934 |
| Gene: |
MCAM |
More about : MCAM |
| Long gene name: |
melanoma cell adhesion molecule |
| Synonyms: |
MUC18 CD146 |
| Synonyms name: |
Gicerin |
| Locus: |
11q23, 3 |
| Discovery year: |
1995-12-18 |
| GenBank acession: |
X68264 |
| Entrez gene record: |
4162 |
| Pubmed identfication: |
2602381 10702685 |
| Classification: |
C2-set domain containing CD molecules V-set domain containing Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000166197 |