| Antigen detectable with this ELISA kit: |
Lymphocyte Activation Gene 3 (LAG3) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
063ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Activation Gene 3 (LAG3), The concentration of Lymphocyte Activation Gene 3 (LAG3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Activation Gene 3 (LAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Lymphocyte Activation Gene 3 (LAG3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Lymphocyte Activation Gene 3 (LAG3) |
| Short name: |
Lymphocyte Activation Gene 3 (LAG3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Lymphocyte Activation Gene 3 (lymphocyte-activation gene 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CD223, Cell surfaces, LAG3 and IDBG-14994 and ENSG00000089692 and 3902, Lag3 and IDBG-188462 and ENSMUSG00000030124 and 16768, MHC class II protein binding, this GO :0003823 and antigen binding and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0042289 and MHC class II protein binding and molecular function this GO :0045085 and negative regulation of interleukin-2 biosynthetic process and biological process this GO :0045954 and positive regulation of natural killer cell mediated cytotoxicity and biological process this GO :0050868 and negative regulation of T cell activation and biological process, this GO :0003823 : antigen binding, this GO :0003823 : antigen binding and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005515 : protein binding and also this GO :0042289 : MHC class II protein binding, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005515 : protein binding, this GO :0042289 : MHC class II protein binding, 18959 and IDBG-640276 and ENSBTAG00000034295 and 515155, lymphocyte-activation gene 3 |
| Identity: |
6476 |
| Gene: |
LAG3 |
More about : LAG3 |
| Long gene name: |
lymphocyte activating 3 |
| Synonyms gene name: |
lymphocyte-activation gene 3 |
| Synonyms: |
CD223 |
| Locus: |
12p13, 31 |
| Discovery year: |
1991-06-03 |
| Entrez gene record: |
3902 |
| RefSeq identity: |
NM_002286 |
| Classification: |
CD molecules Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000169197 |