| Antigen detectable with this ELISA kit: |
Lysosomal Acid (LIPA), Lipase A |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Lysosomal Acid (LIPA), Lysosomal Acid (LIPA), Lysosomal Acid (LIPA), Lysosomal Acid (LIPA) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, The concentration of Lipase A, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Lipase A, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Lysosomal Acid (LIPA), Lipase A |
| Short name: |
Lysosomal Acid (LIPA) ELISA kit, Lipase A |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Lysosomal Acid (lipase A, cholesterol esterase) Enzyme-linked immunosorbent assay reagent, lysosomal acid, Lipase A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CESD and LAL, LIPA and IDBG-81838 and ENSG00000107798 and 101926887, Lipa and IDBG-159667 and ENSMUSG00000024781 and 16889, cholesterol esterase, lipase activity, lysosomal acid, multiple, this GO :0000902 and cell morphogenesis and biological process this GO :0001816 and cytokine production and biological process this GO :0004771 and sterol esterase activity and molecular function this GO :0005764 and lysosome and cellular component this GO :0006629 and lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0008283 and cell proliferation and biological process this GO :0016042 and lipid catabolic process and biological process this GO :0016298 and lipase activity and molecular function this GO :0030324 and lung development and biological process this GO :0048771 and tissue remodeling and biological process this GO :0048873 and homeostasis of number of cells within a tissue and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004771 : sterol esterase activity, this GO :0004771 : sterol esterase activity and also this GO :0016298 : lipase activity, this GO :0016298 : lipase activity, 3988, 65903 and IDBG-637363 and ENSBTAG00000003636 and 100125267, lipase A |
| Identity: |
6617 |
| Gene: |
LIPA |
More about : LIPA |
| Long gene name: |
lipase A, lysosomal acid type |
| Synonyms gene name: |
cholesterol esterase , lipase A, lysosomal acid |
| Synonyms: |
LAL CESD |
| Synonyms name: |
Wolman disease lysosomal acid lipase sterol esterase |
| Locus: |
10q23, 31 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M74775 |
| Entrez gene record: |
3988 |
| Pubmed identfication: |
8432549 |
| RefSeq identity: |
NM_000235 |
| Classification: |
Lipases |
| Havana BLAST/BLAT: |
OTTHUMG00000018716 |