| Antigen detectable with this ELISA kit: |
Erythroid (KLF1), Kruppel Like Factor 1 |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
8pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Erythroid (KLF1), Erythroid (KLF1), Erythroid (KLF1), Erythroid (KLF1) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 1, The concentration of Kruppel Like Factor 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Kruppel Like Factor 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Erythroid (KLF1), Kruppel Like Factor 1 |
| Short name: |
Erythroid (KLF1) ELISA kit, Kruppel Like Factor 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Erythroid (Kruppel-like factor 1 (erythroid)) Enzyme-linked immunosorbent assay reagent, Kruppel Like Factor 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CDAN4 and EKLF and HBFQTL6 and INLU, DNA-templated and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0043249 and erythrocyte maturation and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048821 and erythrocyte development and biological process this GO :1901653 and cellular response to peptide and biological process, KLF1 and IDBG-31252 and ENSG00000105610 and 10661, KLF1 and IDBG-642908 and ENSBTAG00000006083 and 784295, Klf1 and IDBG-175231 and ENSMUSG00000054191 and 16596, metal ion binding, nuclei, this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001889 and liver development and biological process this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005634 and nucleus and cellular component this GO :0006338 and chromatin remodeling and biological process this GO :0006351 and transcription, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, Kruppel-like factor 1 (erythroid) |
| Identity: |
6345 |
| Gene: |
KLF1 |
More about : KLF1 |
| Long gene name: |
Kruppel like factor 1 |
| Synonyms gene name: |
Kruppel-like factor 1 (erythroid) |
| Synonyms: |
EKLF |
| Synonyms name: |
erythroid Kruppel-like factor |
| Locus: |
19p13, 13 |
| Discovery year: |
1999-12-14 |
| GenBank acession: |
U37106 |
| Entrez gene record: |
10661 |
| Pubmed identfication: |
8924208 9119377 |
| RefSeq identity: |
NM_006563 |
| Classification: |
Kruppel like factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000180536 |
| Locus Specific Databases: |
LRG_825 |