| Antigen detectable with this ELISA kit: |
Keratin 18 (KRT18) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
063ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18), The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Keratin 18 (KRT18), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Keratin 18 (KRT18) |
| Short name: |
Keratin 18 (KRT18) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Keratin 18 (keratin 18) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CYK18 and K18, KRT18 and IDBG-35299 and ENSG00000111057 and 3875, KRT18 and IDBG-631626 and ENSBTAG00000001517 and 506480, Krt18 and IDBG-187416 and ENSMUSG00000023043 and 16668, nuclei, scaffold protein binding, this GO :0005198 and structural molecule activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005815 and microtubule organizing center and cellular component this GO :0005882 and intermediate filament and cellular component this GO :0007049 and cell cycle and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016032 and viral process and biological process this GO :0033209 and tumor necrosis factor-mediated signaling pathway and biological process this GO :0034451 and centriolar satellite and cellular component this GO :0043000 and this GO lgi to plasma membrane CFTR protein transport and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045095 and keratin filament and cellular component this GO :0045104 and intermediate filament cytoskeleton organization and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071944 and cell periphery and cellular component this GO :0097110 and scaffold protein binding and molecular function this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097284 and hepatocyte apoptotic process and biological process, this GO :0005198 : structural molecule activity, this GO :0005198 : structural molecule activity and also this GO :0005515 : protein binding and also this GO :0044822 : poly(A) RNA binding and also this GO :0097110 : scaffold protein binding, this GO :0005515 : protein binding, this GO :0044822 : poly(A) RNA binding, this GO :0097110 : scaffold protein binding, keratin 18 |
| Identity: |
6430 |
| Gene: |
KRT18 |
More about : KRT18 |
| Long gene name: |
keratin 18 |
| Synonyms gene name: |
keratin 18, type I |
| Locus: |
12q13, 13 |
| Discovery year: |
1988-08-12 |
| Entrez gene record: |
3875 |
| Pubmed identfication: |
1705144 16831889 |
| RefSeq identity: |
NM_199187 |
| Classification: |
Keratins, type I |
| Havana BLAST/BLAT: |
OTTHUMG00000169882 |