| Antigen detectable with this ELISA kit: |
Keratin 1 (KRT1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
057ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 1 (KRT1), The concentration of Keratin 1 (KRT1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 1 (KRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Keratin 1 (KRT1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Keratin 1 (KRT1) |
| Short name: |
Keratin 1 (KRT1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Keratin 1 (keratin 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CK1 and EHK and EHK1 and EPPK and K1 and KRT1A and NEPPK, KRT1 and IDBG-34896 and ENSG00000167768 and 3848, KRT1 and IDBG-631729 and ENSBTAG00000046089 and 100301161, Krt1 and IDBG-187040 and ENSMUSG00000046834 and 16678, carbohydrate binding, lectin pathway and biological process this GO :0001895 and retina homeostasis and biological process this GO :0004872 and receptor activity and molecular function this GO :0005198 and structural molecule activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005882 and intermediate filament and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006979 and response to oxidative stress and biological process this GO :0016020 and membrane and cellular component this GO :0030246 and carbohydrate binding and molecular function this GO :0042730 and fibrinolysis and biological process this GO :0045095 and keratin filament and cellular component this GO :0045765 and regulation of angiogenesis and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0061436 and establishment of skin barrier and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072562 and blood microparticle and cellular component, nuclei, this GO :0001867 and complement activation, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005198 : structural molecule activity and also this GO :0005515 : protein binding and also this GO :0030246 : carbohydrate binding, this GO :0005198 : structural molecule activity, this GO :0005515 : protein binding, this GO :0030246 : carbohydrate binding, keratin 1 |
| Identity: |
6412 |
| Gene: |
KRT1 |
More about : KRT1 |
| Long gene name: |
keratin 1 |
| Synonyms gene: |
EHK1 |
| Synonyms gene name: |
epidermolytic hyperkeratosis 1 keratin 1, type II |
| Synonyms: |
KRT1A |
| Locus: |
12q13, 13 |
| Discovery year: |
1991-09-12 |
| GenBank acession: |
X69725 |
| Entrez gene record: |
3848 |
| Pubmed identfication: |
2461420 2470667 16831889 |
| RefSeq identity: |
NM_006121 |
| Classification: |
Keratins, type II |
| Havana BLAST/BLAT: |
OTTHUMG00000169749 |