| Antigen detectable with this ELISA kit: |
Interleukin 7 (IL7) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
8pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 7 (IL7), The concentration of Interleukin 7 (IL7) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 7 (IL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 7 (IL7), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
IL7 |
More about : IL7 |
| Gene target: |
Interleukin 7 (IL7) |
| Short name: |
Interleukin 7 (IL7) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 7 (interleukin 7) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IL-7, IL7 and IDBG-26153 and ENSG00000104432 and 3574, IL7 and IDBG-644827 and ENSBTAG00000016284 and 280827, Il7 and IDBG-130583 and ENSMUSG00000040329 and 16196, growth factor activity, this GO :0002360 and T cell lineage commitment and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005139 and interleukin-7 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0006959 and humoral immune response and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043086 and negative regulation of catalytic activity and biological process this GO :0045453 and bone resorption and biological process this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0046622 and positive regulation of organ growth and biological process this GO :0048873 and homeostasis of number of cells within a tissue and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0005139 : interleukin-7 receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005126 : cytokine receptor binding, this GO :0005139 : interleukin-7 receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, interleukin 7 |
| Identity: |
6023 |
| Long gene name: |
interleukin 7 |
| Synonyms: |
IL-7 |
| Locus: |
8q21, 13 |
| Discovery year: |
1989-10-12 |
| GenBank acession: |
J04156 |
| Entrez gene record: |
3574 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000164620 |