| Antigen detectable with this ELISA kit: |
Interleukin 33 (IL33) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 7, 31, 62, 25pg/mL, 5pg/mL, 625pg/mL, 7, 813-500pg/mL The standard curve concentrations used for the ELISA&rsquo, 813pg/mL, s were 500pg/mL |
| Sensitivity: |
7pg/mL, The minimum detectable dose of this kit is typically less than 2 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 33 (IL33), The concentration of Interleukin 33 (IL33) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 33 (IL33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 33 (IL33), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 33 (IL33) |
| Short name: |
Interleukin 33 (IL33) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 33 (interleukin 33) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0030133 and transport vesicle and cellular component this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0032689 and negative regulation of interferon-gamma production and biological process this GO :0032736 and positive regulation of interleukin-13 production and biological process this GO :0032753 and positive regulation of interleukin-4 production and biological process this GO :0032754 and positive regulation of interleukin-5 production and biological process this GO :0032755 and positive regulation of interleukin-6 production and biological process this GO :0043032 and positive regulation of macrophage activation and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0051024 and positive regulation of immunoglobulin secretion and biological process this GO :0051025 and negative regulation of immunoglobulin secretion and biological process this GO :0090197 and positive regulation of chemokine secretion and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process, IL33 and IDBG-48350 and ENSG00000137033 and 90865, IL33 and IDBG-631251 and ENSBTAG00000018347 and 507054, Il33 and IDBG-157638 and ENSMUSG00000024810 and 77125, nuclei, protein binding, this GO :0002686 and negative regulation of leukocyte migration and biological process this GO :0002826 and negative regulation of T-helper 1 type immune response and biological process this GO :0002830 and positive regulation of type 2 immune response and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005694 and chromosome and cellular component this GO :0006351 and transcription, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, interleukin 33 |
| Identity: |
16028 |
| Gene: |
IL33 |
More about : IL33 |
| Long gene name: |
interleukin 33 |
| Synonyms gene: |
C9orf26 |
| Synonyms gene name: |
chromosome 9 open reading frame 26 (NF-HEV) |
| Synonyms: |
DVS27 DKFZp586H0523 NF-HEV IL1F11 |
| Synonyms name: |
DVS27-related protein nuclear factor for high endothelial venules interleukin-1 family, member 11 |
| Locus: |
9p24, 1 |
| Discovery year: |
2003-12-18 |
| GenBank acession: |
AB024518 |
| Entrez gene record: |
90865 |
| Pubmed identfication: |
10566975 12819012 |
| RefSeq identity: |
NM_033439 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000019516 |