| Antigen detectable with this ELISA kit: |
Interleukin 27 (IL27) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
6pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 27 (IL27), The concentration of Interleukin 27 (IL27) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 27 (IL27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 27 (IL27), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 27 (IL27) |
| Short name: |
Interleukin 27 (IL27) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 27 (interleukin 27) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, Extracellular, IL27 and IDBG-22826 and ENSG00000197272 and 246778, Il27 and IDBG-209673 and ENSMUSG00000044701 and 246779, interleukin-27 receptor binding, this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0006954 and inflammatory response and biological process this GO :0009617 and response to bacterium and biological process this GO :0042129 and regulation of T cell proliferation and biological process this GO :0045078 and positive regulation of interferon-gamma biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045523 and interleukin-27 receptor binding and molecular function this GO :0045625 and regulation of T-helper 1 cell differentiation and biological process this GO :0050688 and regulation of defense response to virus and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0045523 : interleukin-27 receptor binding, this GO :0005125 : cytokine activity, this GO :0005515 : protein binding, this GO :0045523 : interleukin-27 receptor binding, 101510 and IDBG-639114 and ENSBTAG00000018015 and 614927, interleukin 27 |
| Identity: |
5984 |
| Gene: |
IL17D |
More about : IL17D |
| Long gene name: |
interleukin 17D |
| Synonyms: |
IL-22 IL-27 IL-17D IL27 FLJ30846 |
| Synonyms name: |
interleukin 27 |
| Locus: |
13q12, 11 |
| Discovery year: |
2000-05-02 |
| GenBank acession: |
AY078238 |
| Entrez gene record: |
53342 |
| Pubmed identfication: |
12097364 |
| RefSeq identity: |
NM_138284 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000016521 |