| Antigen detectable with this ELISA kit: |
Interleukin 21 (IL21) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
1pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 21 (IL21), The concentration of Interleukin 21 (IL21) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 21 (IL21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 21 (IL21), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 21 (IL21) |
| Short name: |
Interleukin 21 (IL21) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 21 (interleukin 21) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IL21 and IDBG-36977 and ENSG00000138684 and 59067, IL21 and IDBG-630309 and ENSBTAG00000012368 and 378475, Il21 and IDBG-140267 and ENSMUSG00000027718 and 60505, interleukin-2 receptor binding, this GO :0002729 and positive regulation of natural killer cell cytokine production and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005134 and interleukin-2 receptor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0032740 and positive regulation of interleukin-17 production and biological process this GO :0032825 and positive regulation of natural killer cell differentiation and biological process this GO :0034105 and positive regulation of tissue remodeling and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0045078 and positive regulation of interferon-gamma biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045954 and positive regulation of natural killer cell mediated cytotoxicity and biological process this GO :0048469 and cell maturation and biological process this GO :0050729 and positive regulation of inflammatory response and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0005134 : interleukin-2 receptor binding, this GO :0005126 : cytokine receptor binding, this GO :0005134 : interleukin-2 receptor binding, interleukin 21 |
| Identity: |
6005 |
| Gene: |
IL21 |
More about : IL21 |
| Long gene name: |
interleukin 21 |
| Synonyms: |
Za11 IL-21 |
| Locus: |
4q27 |
| Discovery year: |
2000-03-29 |
| GenBank acession: |
AF254069 |
| Entrez gene record: |
59067 |
| Pubmed identfication: |
11081504 17947662 |
| RefSeq identity: |
NM_021803 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000133073 |