| Antigen detectable with this ELISA kit: |
Interleukin 17F (IL17F) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17F (IL17F), The concentration of Interleukin 17F (IL17F) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17F (IL17F), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 17F (IL17F), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 17F (IL17F) |
| Short name: |
Interleukin 17F (IL17F) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 17F (interleukin 17F) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IL17F and IDBG-632624 and ENSBTAG00000016835 and 506030, IL17F and IDBG-90517 and ENSG00000112116 and 112744, Il17f and IDBG-136612 and ENSMUSG00000041872 and 257630, protein homodimerization activity, this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006029 and proteoglycan metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0019955 and cytokine binding and molecular function this GO :0042089 and cytokine biosynthetic process and biological process this GO :0042109 and lymphotoxin A biosynthetic process and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0045076 and regulation of interleukin-2 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045408 and regulation of interleukin-6 biosynthetic process and biological process this GO :0045414 and regulation of interleukin-8 biosynthetic process and biological process this GO :0045423 and regulation of granulocyte macrophage colony-stimulating factor biosynthetic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051216 and cartilage development and biological process this GO :1900017 and positive regulation of cytokine production involved in inflammatory response and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0019955 : cytokine binding and also this GO :0042803 : protein homodimerization activity, this GO :0005126 : cytokine receptor binding, this GO :0019955 : cytokine binding, this GO :0042803 : protein homodimerization activity, interleukin 17F |
| Identity: |
16404 |
| Gene: |
IL17F |
More about : IL17F |
| Long gene name: |
interleukin 17F |
| Synonyms: |
IL-17F ML-1 ML1 |
| Locus: |
6p12, 2 |
| Discovery year: |
2002-03-18 |
| GenBank acession: |
AF384857 |
| Entrez gene record: |
112744 |
| Pubmed identfication: |
11591732 11591768 |
| RefSeq identity: |
NM_052872 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000014845 |
| Locus Specific Databases: |
LRG_356 |