| Antigen detectable with this ELISA kit: |
Interleukin 13 (IL13) |
| Species reactivity: |
Canine (Dog), Canis familiaris |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
3pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 13 (IL13), The concentration of Interleukin 13 (IL13) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 13 (IL13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 13 (IL13), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 13 (IL13) |
| Short name: |
Interleukin 13 (IL13) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 13 (interleukin 13) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IL-13 and P600, IL13 and IDBG-42599 and ENSG00000169194 and 3596, IL13 and IDBG-644674 and ENSBTAG00000015953 and 281247, Il13 and IDBG-172064 and ENSMUSG00000020383 and 16163, protein binding, this GO :0001774 and microglial cell activation and biological process this GO :0002639 and positive regulation of immunoglobulin production and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005144 and interleukin-13 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006928 and cellular component movement and biological process this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010155 and regulation of proton transport and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032723 and positive regulation of connective tissue growth factor production and biological process this GO :0033861 and negative regulation of NAD(P)H oxidase activity and biological process this GO :0035094 and response to nicotine and biological process this GO :0042526 and positive regulation of tyrosine phosphorylation of Stat6 protein and biological process this GO :0043032 and positive regulation of macrophage activation and biological process this GO :0043270 and positive regulation of ion transport and biological process this GO :0045087 and innate immune response and biological process this GO :0045471 and response to ethanol and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0050714 and positive regulation of protein secretion and biological process this GO :0051281 and positive regulation of release of sequestered calcium ion into cytosol and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071345 and cellular response to cytokine stimulus and biological process this GO :0071635 and negative regulation of transforming growth factor beta production and biological process this GO :1901215 and negative regulation of neuron death and biological process this GO :1901247 and negative regulation of lung ciliated cell differentiation and biological process this GO :1901251 and positive regulation of lung this GO blet cell differentiation and biological process this GO :2000231 and positive regulation of pancreatic stellate cell proliferation and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0005144 : interleukin-13 receptor binding and also this GO :0005515 : protein binding, this GO :0005126 : cytokine receptor binding, this GO :0005144 : interleukin-13 receptor binding, this GO :0005515 : protein binding, interleukin 13 |
| Identity: |
5973 |
| Gene: |
IL13 |
More about : IL13 |
| Long gene name: |
interleukin 13 |
| Synonyms: |
P600 IL-13 ALRH BHR1 MGC116786 MGC116788 MGC116789 |
| Synonyms name: |
allergic rhinitis Bronchial hyperresponsiveness-1 (bronchial asthma) |
| Locus: |
5q31, 1 |
| Discovery year: |
1993-04-07 |
| GenBank acession: |
U31120 |
| Entrez gene record: |
3596 |
| RefSeq identity: |
NM_002188 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000059723 |