| Antigen detectable with this ELISA kit: |
Interleukin 10 (IL10) |
| Species reactivity: |
Canine (Dog), Canis familiaris |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
6pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10), The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 10 (IL10), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
IL10 |
More about : IL10 |
| Gene target: |
Interleukin 10 (IL10) |
| Short name: |
Interleukin 10 (IL10) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 10 (interleukin 10) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CSIF and GVHDS and IL-10 and IL10A and TGIF, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0051045 and negative regulation of membrane protein ectodomain proteolysis and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051930 and regulation of sensory perception of pain and biological process this GO :0060670 and branching involved in labyrinthine layer morphogenesis and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071392 and cellular response to estradiol stimulus and biological process, Extracellular, IL10 and IDBG-106270 and ENSG00000136634 and 3586, IL10 and IDBG-629159 and ENSBTAG00000006685 and 281246, Il10 and IDBG-190697 and ENSMUSG00000016529 and 16153, growth factor activity, this GO :0001818 and negative regulation of cytokine production and biological process this GO :0002237 and response to molecule of bacterial origin and biological process this GO :0002740 and negative regulation of cytokine secretion involved in immune response and biological process this GO :0002875 and negative regulation of chronic inflammatory response to antigenic stimulus and biological process this GO :0002904 and positive regulation of B cell apoptotic process and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005141 and interleukin-10 receptor binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0007253 and cytoplasmic sequestering of NF-kappaB and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0007568 and aging and biological process this GO :0008083 and growth factor activity and molecular function this GO :0010033 and response to organic substance and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0014823 and response to activity and biological process this GO :0014854 and response to inactivity and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030183 and B cell differentiation and biological process this GO :0030595 and leukocyte chemotaxis and biological process this GO :0030886 and negative regulation of myeloid dendritic cell activation and biological process this GO :0030889 and negative regulation of B cell proliferation and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032689 and negative regulation of interferon-gamma production and biological process this GO :0032695 and negative regulation of interleukin-12 production and biological process this GO :0032715 and negative regulation of interleukin-6 production and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032800 and receptor biosynthetic process and biological process this GO :0032868 and response to insulin and biological process this GO :0034465 and response to carbon monoxide and biological process this GO :0042092 and type 2 immune response and biological process this GO :0042100 and B cell proliferation and biological process this GO :0042130 and negative regulation of T cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0042536 and negative regulation of tumor necrosis factor biosynthetic process and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044130 and negative regulation of growth of symbiont in host and biological process this GO :0045019 and negative regulation of nitric oxide biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045191 and regulation of isotype switching and biological process this GO :0045347 and negative regulation of MHC class II biosynthetic process and biological process this GO :0045348 and positive regulation of MHC class II biosynthetic process and biological process this GO :0045355 and negative regulation of interferon-alpha biosynthetic process and biological process this GO :0045893 and positive regulation of transcription, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005141 : interleukin-10 receptor binding and also this GO :0008083 : growth factor activity, this GO :0005141 : interleukin-10 receptor binding, this GO :0008083 : growth factor activity, interleukin 10 |
| Identity: |
5962 |
| Long gene name: |
interleukin 10 |
| Synonyms: |
CSIF TGIF IL10A IL-10 |
| Synonyms name: |
cytokine synthesis inhibitory factor T-cell growth inhibitory factor |
| Locus: |
1q32, 1 |
| Discovery year: |
1991-10-31 |
| GenBank acession: |
M57627 |
| Entrez gene record: |
3586 |
| Pubmed identfication: |
9162098 |
| RefSeq identity: |
NM_000572 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000036386 |