| Antigen detectable with this ELISA kit: |
Interleukin 1 Receptor Associated Kinase 4 (IRAK4) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
54ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4), The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 1 Receptor Associated Kinase 4 (IRAK4) |
| Short name: |
Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 1 Receptor Associated phosphorylation catalyst 4 (interleukin-1 receptor-associated kinase 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IRAK4 and IDBG-28022 and ENSG00000198001 and 51135, IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692, Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632, this GO :0000287 and magnesium ion binding and molecular function this GO :0001816 and cytokine production and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005149 and interleukin-1 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007254 and JNK cascade and biological process this GO :0010008 and endosome membrane and cellular component this GO :0016772 and transferase activity, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005149 : interleukin-1 receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005149 : interleukin-1 receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045087 and innate immune response and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process, interleukin-1 receptor-associated kinase 4 |
| Identity: |
17967 |
| Gene: |
IRAK4 |
More about : IRAK4 |
| Long gene name: |
interleukin 1 receptor associated kinase 4 |
| Synonyms: |
NY-REN-64 |
| Locus: |
12q12 |
| Discovery year: |
2002-09-27 |
| GenBank acession: |
AF155118 |
| Entrez gene record: |
51135 |
| Pubmed identfication: |
3772297 10508479 |
| Havana BLAST/BLAT: |
OTTHUMG00000169425 |
| Locus Specific Databases: |
IRAK4base: Mutation registry for IRAK4 deficiency LRG_75 |