| Antigen detectable with this ELISA kit: |
Insulin Receptor Substrate 2 (IRS2) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
053ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 2 (IRS2), The concentration of Insulin Receptor Substrate 2 (IRS2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 2 (IRS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Insulin Receptor Substrate 2 (IRS2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Insulin Receptor Substrate 2 (IRS2) |
| Short name: |
Insulin Receptor Substrate 2 (IRS2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Insulin Receptor Substrate 2 (insulin receptor substrate 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
14-3-3 protein binding, IRS-2, IRS2 and IDBG-50213 and ENSG00000185950 and 8660, IRS2 and IDBG-633698 and ENSBTAG00000012019 and 100300012, Irs2 and IDBG-132183 and ENSMUSG00000038894 and 384783, Plasma membranes, this GO :0002053 and positive regulation of mesenchymal cell proliferation and biological process this GO :0002903 and negative regulation of B cell apoptotic process and biological process this GO :0004871 and signal transducer activity and molecular function this GO :0005158 and insulin receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006006 and glucose metabolic process and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007420 and brain development and biological process this GO :0008283 and cell proliferation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009749 and response to glucose and biological process this GO :0010748 and negative regulation of plasma membrane long-chain fatty acid transport and biological process this GO :0010907 and positive regulation of glucose metabolic process and biological process this GO :0019216 and regulation of lipid metabolic process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0019903 and protein phosphatase binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0030335 and positive regulation of cell migration and biological process this GO :0030879 and mammary gland development and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0032000 and positive regulation of fatty acid beta-oxidation and biological process this GO :0032024 and positive regulation of insulin secretion and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0033673 and negative regulation of kinase activity and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0043548 and phosphatidylinositol 3-kinase binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0055088 and lipid homeostasis and biological process this GO :0060397 and JAK-STAT cascade involved in growth hormone signaling pathway and biological process this GO :0071333 and cellular response to glucose stimulus and biological process this GO :0071889 and 14-3-3 protein binding and molecular function this GO :1901653 and cellular response to peptide and biological process, this GO :0004871 : signal transducer activity, this GO :0004871 : signal transducer activity and also this GO :0005158 : insulin receptor binding and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding and also this GO :0019903 : protein phosphatase binding and also this GO :0019904 : protein domain specific binding and also this GO :0043548 : phosphatidylinositol 3-kinase binding and also this GO :0071889 : 14-3-3 protein binding, this GO :0005158 : insulin receptor binding, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, this GO :0019903 : protein phosphatase binding, this GO :0019904 : protein domain specific binding, this GO :0043548 : phosphatidylinositol 3-kinase binding, this GO :0071889 : 14-3-3 protein binding, insulin receptor substrate 2 |
| Identity: |
6126 |
| Gene: |
IRS2 |
More about : IRS2 |
| Long gene name: |
insulin receptor substrate 2 |
| Locus: |
13q34 |
| Discovery year: |
1998-11-24 |
| GenBank acession: |
AB000732 |
| Entrez gene record: |
8660 |
| Pubmed identfication: |
9312143 |
| RefSeq identity: |
NM_003749 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000017338 |