| Antigen detectable with this ELISA kit: |
Insulin Degrading Enzyme (IDE) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 2000pg/mL, 250pg/mL, 500pg/mL, 62, 5-4000pg/mL The standard curve concentrations used for the ELISA&rsquo, 5pg/mL, 62, s were 4000pg/mL |
| Sensitivity: |
8pg/mL, The minimum detectable dose of this kit is typically less than 26 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE), The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Insulin Degrading Enzyme (IDE), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Activating enzymes will cut off the domain that is biological active to become functional, If the substrate is DNA they are called restriction enzymes, Enzymes are cleaving the substrate |
| Gene target: |
Insulin Degrading (IDE) |
| Short name: |
Insulin Degrading Enzyme (IDE) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, enzymes, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Insulin Degrading Enzyme (insulin-degrading enzyme) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
enzymes |
| Alternative to gene target: |
IDE and IDBG-637578 and ENSBTAG00000019759 and 523752, IDE and IDBG-82456 and ENSG00000119912 and 3416, INSULYSIN, Ide and IDBG-161417 and ENSMUSG00000056999 and 15925, metal ion binding, nuclei, this GO :0001540 and beta-amyloid binding and molecular function this GO :0001948 and glycoprotein binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004222 and metalloendopeptidase activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005777 and peroxisome and cellular component this GO :0005782 and peroxisomal matrix and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006508 and proteolysis and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008340 and determination of adult lifespan and biological process this GO :0009986 and cell surface and cellular component this GO :0010815 and bradykinin catabolic process and biological process this GO :0010992 and ubiquitin homeostasis and biological process this GO :0016032 and viral process and biological process this GO :0016887 and ATPase activity and molecular function this GO :0017046 and peptide hormone binding and molecular function this GO :0031597 and cytosolic proteasome complex and cellular component this GO :0031626 and beta-endorphin binding and molecular function this GO :0032461 and positive regulation of protein oli this GO merization and biological process this GO :0042277 and peptide binding and molecular function this GO :0042447 and hormone catabolic process and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043130 and ubiquitin binding and molecular function this GO :0043559 and insulin binding and molecular function this GO :0044257 and cellular protein catabolic process and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050435 and beta-amyloid metabolic process and biological process this GO :0051260 and protein homooli this GO merization and biological process this GO :0051289 and protein homotetramerization and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051603 and proteolysis involved in cellular protein catabolic process and biological process this GO :1901142 and insulin metabolic process and biological process this GO :1901143 and insulin catabolic process and biological process, this GO :0001540 : beta-amyloid binding, this GO :0001540 : beta-amyloid binding and also this GO :0001948 : glycoprotein binding and also this GO :0003824 : catalytic activity and also this GO :0004222 : metalloendopeptidase activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008270 : zinc ion binding and also this GO :0016887 : ATPase activity and also this GO :0017046 : peptide hormone binding and also this GO :0031626 : beta-endorphin binding and also this GO :0042277 : peptide binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043130 : ubiquitin binding and also this GO :0043559 : insulin binding and also this GO :0046872 : metal ion binding, this GO :0001948 : glycoprotein binding, this GO :0003824 : catalytic activity, this GO :0004222 : metalloendopeptidase activity, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008270 : zinc ion binding, this GO :0016887 : ATPase activity, this GO :0017046 : peptide hormone binding, this GO :0031626 : beta-endorphin binding, this GO :0042277 : peptide binding, this GO :0042803 : protein homodimerization activity, this GO :0043130 : ubiquitin binding, this GO :0043559 : insulin binding, this GO :0046872 : metal ion binding, insulin-degrading enzyme |
| Identity: |
5381 |
| Gene: |
IDE |
More about : IDE |
| Long gene name: |
insulin degrading enzyme |
| Synonyms gene name: |
insulin-degrading enzyme |
| Synonyms name: |
insulysin |
| Locus: |
10q23, 33 |
| Discovery year: |
1990-07-03 |
| GenBank acession: |
M21188 |
| Entrez gene record: |
3416 |
| Pubmed identfication: |
2293021 |
| RefSeq identity: |
NM_004969 |
| Havana BLAST/BLAT: |
OTTHUMG00000018759 |