| Antigen detectable with this ELISA kit: |
Immediate Early Response 3 (IER3) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immediate Early Response 3 (IER3), The concentration of Immediate Early Response 3 (IER3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immediate Early Response 3 (IER3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Immediate Early Response 3 (IER3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Immediate Early Response 3 (IER3) |
| Short name: |
Immediate Early Response 3 (IER3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Immediate Early Response 3 (immediate early response 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DIF-2 and DIF2 and GLY96 and IEX-1 and IEX-1L and IEX1 and PRG1, IER3 and IDBG-634508 and ENSBTAG00000011358 and 505455, IER3 and IDBG-76842 and ENSG00000137331 and 8870, Ier3 and IDBG-179064 and ENSMUSG00000003541 and 15937, nuclei, protein binding, this GO :0001562 and response to protozoan and biological process this GO :0003085 and negative regulation of systemic arterial blood pressure and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006282 and regulation of DNA repair and biological process this GO :0006915 and apoptotic process and biological process this GO :0007095 and mitotic G2 DNA damage checkpoint and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0045820 and negative regulation of glycolytic process and biological process this GO :0046822 and regulation of nucleocytoplasmic transport and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :1901029 and negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway and biological process this GO :2000377 and regulation of reactive oxygen species metabolic process and biological process this GO :2001020 and regulation of response to DNA damage stimulus and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, immediate early response 3 |
| Identity: |
5392 |
| Gene: |
IER3 |
More about : IER3 |
| Long gene name: |
immediate early response 3 |
| Synonyms: |
IEX-1 DIF-2 PRG1 IEX-1L |
| Locus: |
6p21, 33 |
| Discovery year: |
1999-09-30 |
| GenBank acession: |
AF083421 |
| Entrez gene record: |
8870 |
| Pubmed identfication: |
8603392 9703517 |
| Havana BLAST/BLAT: |
OTTHUMG00000031265 |