| Antigen detectable with this ELISA kit: |
IL2 Inducible T-Cell Kinase (ITK) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
107ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL2 Inducible T-Cell Kinase (ITK), The concentration of IL2 Inducible T-Cell Kinase (ITK) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL2 Inducible T-Cell Kinase (ITK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain IL2 Inducible T-Cell Kinase (ITK), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
ITK |
More about : ITK |
| Gene target: |
IL2 T Kinase (ITK) |
| Short name: |
IL2 T-Cell Kinase (ITK) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
interleukin 2 Inducible T-cellular phosphorylation catalyst (interleukin 2-inducible T-cell kinase) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cell surfaces, EMT and LPFS1 and LYK and PSCTK2, Extracellular, IL-2 and lymphokine and TCGF, IL2 and IDBG-36892 and ENSG00000109471 and 3558, IL2 and IDBG-630322 and ENSBTAG00000020883 and 280822, IL2-inducible T-cell kinase, ITK and IDBG-55348 and ENSG00000113263 and 3702, ITK and IDBG-646384 and ENSBTAG00000000306 and 522311, Il2 and IDBG-140190 and ENSMUSG00000027720 and 16183, Itk and IDBG-165583 and ENSMUSG00000020395 and 16428, glycosphin this GO lipid binding, this GO :0001816 and cytokine production and biological process this GO :0001865 and NK T cell differentiation and biological process this GO :0002250 and adaptive immune response and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006968 and cellular defense response and biological process this GO :0007165 and signal transduction and biological process this GO :0007202 and activation of phospholipase C activity and biological process this GO :0016772 and transferase activity, this GO :0001933 and negative regulation of protein phosphorylation and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002903 and negative regulation of B cell apoptotic process and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005134 and interleukin-2 receptor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0019209 and kinase activator activity and molecular function this GO :0030101 and natural killer cell activation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0030307 and positive regulation of cell growth and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031851 and kappa-type opioid receptor binding and molecular function this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032740 and positive regulation of interleukin-17 production and biological process this GO :0034105 and positive regulation of tissue remodeling and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042104 and positive regulation of activated T cell proliferation and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043208 and glycosphin this GO lipid binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0045591 and positive regulation of regulatory T cell differentiation and biological process this GO :0045822 and negative regulation of heart contraction and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046013 and regulation of T cell homeostatic proliferation and biological process this GO :0048304 and positive regulation of isotype switching to IgG isotypes and biological process this GO :0050672 and negative regulation of lymphocyte proliferation and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0051024 and positive regulation of immunoglobulin secretion and biological process this GO :0060999 and positive regulation of dendritic spine development and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005134 : interleukin-2 receptor binding and also this GO :0008083 : growth factor activity and also this GO :0019209 : kinase activator activity and also this GO :0030246 : carbohydrate binding and also this GO :0031851 : kappa-type opioid receptor binding and also this GO :0043208 : glycosphin this GO lipid binding, this GO :0005134 : interleukin-2 receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008083 : growth factor activity, this GO :0016772 : transferase activity, this GO :0019209 : kinase activator activity, this GO :0030246 : carbohydrate binding, this GO :0031851 : kappa-type opioid receptor binding, this GO :0043208 : glycosphin this GO lipid binding, this GO :0046872 : metal ion binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0032609 and interferon-gamma production and biological process this GO :0032633 and interleukin-4 production and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042110 and T cell activation and biological process this GO :0045087 and innate immune response and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050852 and T cell receptor signaling pathway and biological process, interleukin 2 |
| Identity: |
6171 |
| Long gene name: |
IL2 inducible T-cell kinase |
| Synonyms gene name: |
IL2-inducible T-cell kinase |
| Synonyms: |
EMT PSCTK2 LYK |
| Locus: |
5q33, 3 |
| Discovery year: |
1995-12-18 |
| GenBank acession: |
D13720 |
| Entrez gene record: |
3702 |
| Pubmed identfication: |
8364206 |
| Classification: |
Pleckstrin homology domain containing SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130245 |
| Locus Specific Databases: |
LRG_189 |