| Antigen detectable with this ELISA kit: |
Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
061ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), The concentration of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) |
| Short name: |
Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Hypoxanthine Phosphoribosyltransferase 1 (hypoxanthine phosphoribosyltransferase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cytoplasm, HGPRT and HPRT, HPRT1 and IDBG-86864 and ENSG00000165704 and 3251, Hprt and IDBG-145409 and ENSMUSG00000025630 and 15452, LOC510369 and IDBG-640500 and ENSBTAG00000046220 and 510369, guanine phosphoribosyltransferase activity, this GO :0000166 and nucleotide binding and molecular function this GO :0000287 and magnesium ion binding and molecular function this GO :0001975 and response to amphetamine and biological process this GO :0004422 and hypoxanthine phosphoribosyltransferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006144 and purine nucleobase metabolic process and biological process this GO :0006164 and purine nucleotide biosynthetic process and biological process this GO :0006166 and purine ribonucleoside salvage and biological process this GO :0006168 and adenine salvage and biological process this GO :0006178 and guanine salvage and biological process this GO :0007625 and grooming behavior and biological process this GO :0007626 and locomotory behavior and biological process this GO :0009116 and nucleoside metabolic process and biological process this GO :0019835 and cytolysis and biological process this GO :0021756 and striatum development and biological process this GO :0021895 and cerebral cortex neuron differentiation and biological process this GO :0021954 and central nervous system neuron development and biological process this GO :0032263 and GMP salvage and biological process this GO :0032264 and IMP salvage and biological process this GO :0042417 and dopamine metabolic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043101 and purine-containing compound salvage and biological process this GO :0043103 and hypoxanthine salvage and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045964 and positive regulation of dopamine metabolic process and biological process this GO :0046038 and GMP catabolic process and biological process this GO :0046040 and IMP metabolic process and biological process this GO :0046083 and adenine metabolic process and biological process this GO :0046100 and hypoxanthine metabolic process and biological process this GO :0046651 and lymphocyte proliferation and biological process this GO :0048813 and dendrite morphogenesis and biological process this GO :0051289 and protein homotetramerization and biological process this GO :0052657 and guanine phosphoribosyltransferase activity and molecular function this GO :0055086 and nucleobase-containing small molecule metabolic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0000287 : magnesium ion binding and also this GO :0004422 : hypoxanthine phosphoribosyltransferase activity and also this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0052657 : guanine phosphoribosyltransferase activity, this GO :0000287 : magnesium ion binding, this GO :0004422 : hypoxanthine phosphoribosyltransferase activity, this GO :0005515 : protein binding, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0052657 : guanine phosphoribosyltransferase activity, hypoxanthine phosphoribosyltransferase 1 |
| Identity: |
5157 |
| Gene: |
HPRT1 |
More about : HPRT1 |
| Long gene name: |
hypoxanthine phosphoribosyltransferase 1 |
| Synonyms gene: |
HPRT |
| Synonyms: |
HGPRT |
| Synonyms name: |
Lesch-Nyhan syndrome |
| Locus: |
Xq26, 2-q26, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M26434 |
| Entrez gene record: |
3251 |
| Pubmed identfication: |
12175903 |
| RefSeq identity: |
NM_000194 |
| Havana BLAST/BLAT: |
OTTHUMG00000022452 |
| Locus Specific Databases: |
Hypoxanthine Guanine Phosphoribosyltransferase 1, Lech-Nyhan Syndrome |