| Antigen detectable with this ELISA kit: |
Histone Deacetylase 5 (HDAC5) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
113ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 5 (HDAC5), The concentration of Histone Deacetylase 5 (HDAC5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 5 (HDAC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Histone Deacetylase 5 (HDAC5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Histone Deacetylase 5 (HDAC5) |
| Short name: |
Histone Deacetylase 5 (HDAC5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Histone Deacetylase 5 (histone deacetylase 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006954 and inflammatory response and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007507 and heart development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0010830 and regulation of myotube differentiation and biological process this GO :0010832 and negative regulation of myotube differentiation and biological process this GO :0016568 and chromatin modification and biological process this GO :0016575 and histone deacetylation and biological process this GO :0016604 and nuclear body and cellular component this GO :0030183 and B cell differentiation and biological process this GO :0032041 and NAD-dependent histone deacetylase activity (H3-K14 specific) and molecular function this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0033555 and multicellular organismal response to stress and biological process this GO :0042113 and B cell activation and biological process this GO :0042220 and response to cocaine and biological process this GO :0042493 and response to drug and biological process this GO :0043234 and protein complex and cellular component this GO :0043393 and regulation of protein binding and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0046969 and NAD-dependent histone deacetylase activity (H3-K9 specific) and molecular function this GO :0046970 and NAD-dependent histone deacetylase activity (H4-K16 specific) and molecular function this GO :0048742 and regulation of skeletal muscle fiber development and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051153 and regulation of striated muscle cell differentiation and biological process this GO :0070491 and repressing transcription factor binding and molecular function this GO :0070932 and histone H3 deacetylation and biological process this GO :0070933 and histone H4 deacetylation and biological process this GO :0090051 and negative regulation of cell migration involved in sprouting angiogenesis and biological process this GO :0097372 and NAD-dependent histone deacetylase activity (H3-K18 specific) and molecular function, HD5 and NY-CO-9, HDAC5 and IDBG-53295 and ENSG00000108840 and 10014, HDAC5 and IDBG-640847 and ENSBTAG00000016254 and 504242, Hdac5 and IDBG-212520 and ENSMUSG00000008855 and 15184, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, this GO :0000118 and histone deacetylase complex and cellular component this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0002076 and osteoblast development and biological process this GO :0003714 and transcription corepressor activity and molecular function this GO :0004407 and histone deacetylase activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006325 and chromatin organization and biological process this GO :0006338 and chromatin remodeling and biological process this GO :0006342 and chromatin silencing and biological process this GO :0006351 and transcription, this GO :0003714 : transcription corepressor activity, this GO :0003714 : transcription corepressor activity and also this GO :0004407 : histone deacetylase activity and also this GO :0005080 : protein kinase C binding and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0032041 : NAD-dependent histone deacetylase activity (H3-K14 specific) and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding and also this GO :0046969 : NAD-dependent histone deacetylase activity (H3-K9 specific) and also this GO :0046970 : NAD-dependent histone deacetylase activity (H4-K16 specific) and also this GO :0070491 : repressing transcription factor binding and also this GO :0097372 : NAD-dependent histone deacetylase activity (H3-K18 specific), this GO :0004407 : histone deacetylase activity, this GO :0005080 : protein kinase C binding, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0032041 : NAD-dependent histone deacetylase activity (H3-K14 specific), this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, this GO :0046969 : NAD-dependent histone deacetylase activity (H3-K9 specific), this GO :0046970 : NAD-dependent histone deacetylase activity (H4-K16 specific), this GO :0070491 : repressing transcription factor binding, this GO :0097372 : NAD-dependent histone deacetylase activity (H3-K18 specific), histone deacetylase 5 |
| Identity: |
14068 |
| Gene: |
HDAC5 |
More about : HDAC5 |
| Long gene name: |
histone deacetylase 5 |
| Synonyms: |
KIAA0600 NY-CO-9 FLJ90614 |
| Locus: |
17q21, 31 |
| Discovery year: |
2000-11-28 |
| GenBank acession: |
AF249731 |
| Entrez gene record: |
10014 |
| Pubmed identfication: |
10220385 9610721 |
| RefSeq identity: |
NM_001015053 |
| Classification: |
Histone deacetylases, class IIA |
| Havana BLAST/BLAT: |
OTTHUMG00000181806 |