| Antigen detectable with this ELISA kit: |
Heat Shock 70kDa Protein 5 (HSPA5) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5), The concentration of Heat Shock 70kDa Protein 5 (HSPA5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Heat Shock 70kDa Protein 5 (HSPA5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Heat Shock 70kDa Protein 5 (HSPA5) |
| Short name: |
Heat Shock 70kDa Protein 5 (HSPA5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
78kDa)) Enzyme-linked immunosorbent assay reagent, Heat Shock 70kDa Protein 5 (heat shock 70kDa protein 5 (glucose-regulated protein |
| Alternative technique: |
elisas |
| Alternative to gene target: |
78kDa), BIP and GRP78 and HEL-S-89n and MIF2, HSPA5 and IDBG-85148 and ENSG00000044574 and 3309, Hspa5 and IDBG-163151 and ENSMUSG00000026864 and 14828, misfolded protein binding, nuclei, this GO :0000902 and cell morphogenesis and biological process this GO :0001948 and glycoprotein binding and molecular function this GO :0002576 and platelet degranulation and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005790 and smooth endoplasmic reticulum and cellular component this GO :0005793 and endoplasmic reticulum- this GO lgi intermediate compartment and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006983 and ER overload response and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007596 and blood coagulation and biological process this GO :0008180 and COP9 signalosome and cellular component this GO :0009986 and cell surface and cellular component this GO :0016020 and membrane and cellular component this GO :0016887 and ATPase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0021589 and cerebellum structural organization and biological process this GO :0021680 and cerebellar Purkinje cell layer development and biological process this GO :0021762 and substantia nigra development and biological process this GO :0030168 and platelet activation and biological process this GO :0030176 and integral component of endoplasmic reticulum membrane and cellular component this GO :0030335 and positive regulation of cell migration and biological process this GO :0030433 and ER-associated ubiquitin-dependent protein catabolic process and biological process this GO :0030496 and midbody and cellular component this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0034663 and endoplasmic reticulum chaperone complex and cellular component this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0035437 and maintenance of protein localization in endoplasmic reticulum and biological process this GO :0042149 and cellular response to glucose starvation and biological process this GO :0042470 and melanosome and cellular component this GO :0043022 and ribosome binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0051082 and unfolded protein binding and molecular function this GO :0051087 and chaperone binding and molecular function this GO :0051603 and proteolysis involved in cellular protein catabolic process and biological process this GO :0051787 and misfolded protein binding and molecular function this GO :0060904 and regulation of protein folding in endoplasmic reticulum and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071236 and cellular response to antibiotic and biological process this GO :0071353 and cellular response to interleukin-4 and biological process, this GO :0001948 : glycoprotein binding, this GO :0001948 : glycoprotein binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016887 : ATPase activity and also this GO :0019899 : enzyme binding and also this GO :0019904 : protein domain specific binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0043022 : ribosome binding and also this GO :0051082 : unfolded protein binding and also this GO :0051087 : chaperone binding and also this GO :0051787 : misfolded protein binding, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016887 : ATPase activity, this GO :0019899 : enzyme binding, this GO :0019904 : protein domain specific binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0043022 : ribosome binding, this GO :0051082 : unfolded protein binding, this GO :0051087 : chaperone binding, this GO :0051787 : misfolded protein binding, heat shock 70kDa protein 5 (glucose-regulated protein |
| Identity: |
5238 |
| Gene: |
HSPA5 |
More about : HSPA5 |
| Long gene name: |
heat shock protein family A (Hsp70) member 5 |
| Synonyms gene: |
GRP78 |
| Synonyms gene name: |
78kD) heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) , heat shock 70kD protein 5 (glucose-regulated protein |
| Synonyms: |
BiP |
| Synonyms name: |
78kDa , glucose-regulated protein |
| Locus: |
9q33, 3 |
| Discovery year: |
1991-07-26 |
| Entrez gene record: |
3309 |
| Classification: |
Heat shock 70kDa proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000020672 |