| Antigen detectable with this ELISA kit: |
Heat Shock 70kDa Protein 1B (HSPA1B) |
| Species reactivity: |
Bovine (Cattle), Bos taurus |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
53ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B), The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Heat Shock 70kDa Protein 1B (HSPA1B) |
| Short name: |
Heat Shock 70kDa Protein 1B (HSPA1B) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Heat Shock 70kDa Protein 1B (heat shock 70kDa protein 1B) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ATPase activity, HEL-S-103 and HSP70-1 and HSP70-1A and HSP70-1B and HSP70-2 and HSP70I and HSP72 and HSPA1, HSPA1B and IDBG-79309 and ENSG00000204388 and 3303, coupled, coupled and also this GO :0044183 : protein binding involved in protein folding and also this GO :0044822 : poly(A) RNA binding and also this GO :0047485 : protein N-terminus binding and also this GO :0051082 : unfolded protein binding, coupled and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044183 and protein binding involved in protein folding and molecular function this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045648 and positive regulation of erythrocyte differentiation and biological process this GO :0047485 and protein N-terminus binding and molecular function this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051082 and unfolded protein binding and molecular function this GO :0060548 and negative regulation of cell death and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070370 and cellular heat acclimation and biological process this GO :0072562 and blood microparticle and cellular component this GO :0090084 and negative regulation of inclusion body assembly and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, nuclei, this GO :0000902 and cell morphogenesis and biological process this GO :0001618 and virus receptor activity and molecular function this GO :0003725 and double-stranded RNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005814 and centriole and cellular component this GO :0005829 and cytosol and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006402 and mRNA catabolic process and biological process this GO :0006986 and response to unfolded protein and biological process this GO :0008180 and COP9 signalosome and cellular component this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009615 and response to virus and biological process this GO :0010467 and gene expression and biological process this GO :0016070 and RNA metabolic process and biological process this GO :0016071 and mRNA metabolic process and biological process this GO :0016234 and inclusion body and cellular component this GO :0016235 and aggresome and cellular component this GO :0016607 and nuclear speck and cellular component this GO :0016887 and ATPase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0030308 and negative regulation of cell growth and biological process this GO :0030529 and ribonucleoprotein complex and cellular component this GO :0031072 and heat shock protein binding and molecular function this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0034605 and cellular response to heat and biological process this GO :0042026 and protein refolding and biological process this GO :0042623 and ATPase activity, this GO :0001618 : virus receptor activity, this GO :0001618 : virus receptor activity and also this GO :0003725 : double-stranded RNA binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016887 : ATPase activity and also this GO :0019899 : enzyme binding and also this GO :0031072 : heat shock protein binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0042623 : ATPase activity, this GO :0003725 : double-stranded RNA binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016887 : ATPase activity, this GO :0019899 : enzyme binding, this GO :0031072 : heat shock protein binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0042623 : ATPase activity, this GO :0044183 : protein binding involved in protein folding, this GO :0044822 : poly(A) RNA binding, this GO :0047485 : protein N-terminus binding, this GO :0051082 : unfolded protein binding, 3304, heat shock 70kDa protein 1B |
| Identity: |
5233 |
| Gene: |
HSPA1B |
More about : HSPA1B |
| Long gene name: |
heat shock protein family A (Hsp70) member 1B |
| Synonyms gene name: |
heat shock 70kD protein 1B heat shock 70kDa protein 1B |
| Synonyms: |
HSP70-2 |
| Locus: |
6p21, 33 |
| Discovery year: |
1995-08-11 |
| Entrez gene record: |
3304 |
| Pubmed identfication: |
1700760 |
| Classification: |
Heat shock 70kDa proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000031202 |