| Antigen detectable with this ELISA kit: |
Growth Associated Protein 43 (GAP43) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
056ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Associated Protein 43 (GAP43), The concentration of Growth Associated Protein 43 (GAP43) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Associated Protein 43 (GAP43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Growth Associated Protein 43 (GAP43), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Growth Associated Protein 43 (GAP43) |
| Short name: |
Growth Associated Protein 43 (GAP43) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Growth Associated Protein 43 (growth associated protein 43) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
B-50 and PP46, Cell surfaces, GAP43 and IDBG-50973 and ENSG00000172020 and 2596, GAP43 and IDBG-632650 and ENSBTAG00000006451 and 281777, Gap43 and IDBG-160941 and ENSMUSG00000047261 and 14432, calmodulin binding, this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0007399 and nervous system development and biological process this GO :0007411 and axon guidance and biological process this GO :0009611 and response to wounding and biological process this GO :0010001 and glial cell differentiation and biological process this GO :0016198 and axon choice point recognition and biological process this GO :0030054 and cell junction and cellular component this GO :0030424 and axon and cellular component this GO :0031527 and filopodium membrane and cellular component this GO :0032584 and growth cone membrane and cellular component this GO :0040008 and regulation of growth and biological process this GO :0042246 and tissue regeneration and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045202 and synapse and cellular component this GO :0051489 and regulation of filopodium assembly and biological process this GO :0071944 and cell periphery and cellular component, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding, this GO :0005516 : calmodulin binding, growth associated protein 43 |
| Identity: |
4140 |
| Gene: |
GAP43 |
More about : GAP43 |
| Long gene name: |
growth associated protein 43 |
| Synonyms: |
B-50 PP46 |
| Synonyms name: |
neuron growth-associated protein 43 neuromodulin nerve growth-related peptide GAP43 axonal membrane protein GAP-43 protein F1 calmodulin-binding protein P-57 neural phosphoprotein B-50 |
| Locus: |
3q13, 31 |
| Discovery year: |
1990-07-10 |
| Entrez gene record: |
2596 |
| Pubmed identfication: |
3272162 8231732 |
| RefSeq identity: |
NM_002045 |
| Havana BLAST/BLAT: |
OTTHUMG00000133758 |