| Antigen detectable with this ELISA kit: |
Granzyme B (GZMB) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
3pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme B (GZMB), The concentration of Granzyme B (GZMB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme B (GZMB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Granzyme B (GZMB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Granzyme B (GZMB) |
| Short name: |
Granzyme B (GZMB) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
cytotoxic T-lymphocyte-associated serine esterase 1)) Enzyme-linked immunosorbent assay reagent, Granzyme B (granzyme B (granzyme 2 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CCPI and CGL-1 and CGL1 and CSP-B and CSPB and CTLA1 and CTSGL1 and HLP and SECT, GZMB and IDBG-4054 and ENSG00000100453 and 3002, cytotoxic T-lymphocyte-associated serine esterase 1), nuclei, serine-type peptidase activity, this GO :0001772 and immunological synapse and cellular component this GO :0003824 and catalytic activity and molecular function this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006915 and apoptotic process and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0008236 and serine-type peptidase activity and molecular function this GO :0008626 and granzyme-mediated apoptotic signaling pathway and biological process this GO :0016020 and membrane and cellular component this GO :0019835 and cytolysis and biological process this GO :0030141 and secretory granule and cellular component this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004252 : serine-type endopeptidase activity and also this GO :0005515 : protein binding and also this GO :0008236 : serine-type peptidase activity, this GO :0004252 : serine-type endopeptidase activity, this GO :0005515 : protein binding, this GO :0008236 : serine-type peptidase activity, 19365 and IDBG-632083 and ENSBTAG00000010057 and 508646, granzyme B (granzyme 2 |
| Identity: |
4709 |
| Gene: |
GZMB |
More about : GZMB |
| Long gene name: |
granzyme B |
| Synonyms gene: |
CTLA1 CSPB |
| Synonyms gene name: |
cytotoxic T-lymphocyte-associated serine esterase 1) , granzyme B (granzyme 2 |
| Synonyms: |
CCPI CGL-1 CSP-B CGL1 CTSGL1 HLP SECT |
| Synonyms name: |
fragmentin 2 cytotoxic serine protease B cathepsin G-like 1 T-cell serine protease 1-3E granzyme 2 cytotoxic T-lymphocyte-associated serine esterase 1 |
| Locus: |
14q12 |
| Discovery year: |
1989-05-12 |
| GenBank acession: |
BC030195 |
| Entrez gene record: |
3002 |
| Pubmed identfication: |
2323780 |
| RefSeq identity: |
NM_004131 |
| Havana BLAST/BLAT: |
OTTHUMG00000029369 |