| Antigen detectable with this ELISA kit: |
Glypican 3 (GPC3) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glypican 3 (GPC3), The concentration of Glypican 3 (GPC3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glypican 3 (GPC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Glypican 3 (GPC3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Glypican 3 (GPC3) |
| Short name: |
Glypican 3 (GPC3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Glypican 3 (glypican 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DGSX and GTR2-2 and MXR7 and OCI-5 and SDYS and SGB and SGBS and SGBS1, Extracellular, GPC3 and IDBG-630953 and ENSBTAG00000020406 and 615239, GPC3 and IDBG-86754 and ENSG00000147257 and 2719, Gpc3 and IDBG-145213 and ENSMUSG00000055653 and 14734, peptidyl-dipeptidase inhibitor activity, planar cell polarity pathway and biological process, this GO :0001523 and retinoid metabolic process and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001822 and kidney development and biological process this GO :0005515 and protein binding and molecular function this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005764 and lysosome and cellular component this GO :0005796 and this GO lgi lumen and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006024 and glycosaminoglycan biosynthetic process and biological process this GO :0006027 and glycosaminoglycan catabolic process and biological process this GO :0007603 and phototransduction, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0043395 : heparan sulfate proteoglycan binding and also this GO :0060422 : peptidyl-dipeptidase inhibitor activity, this GO :0043395 : heparan sulfate proteoglycan binding, this GO :0060422 : peptidyl-dipeptidase inhibitor activity, visible light and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0009948 and anterior/posterior axis specification and biological process this GO :0010171 and body morphogenesis and biological process this GO :0010466 and negative regulation of peptidase activity and biological process this GO :0016020 and membrane and cellular component this GO :0030203 and glycosaminoglycan metabolic process and biological process this GO :0030204 and chondroitin sulfate metabolic process and biological process this GO :0030282 and bone mineralization and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0030324 and lung development and biological process this GO :0030513 and positive regulation of BMP signaling pathway and biological process this GO :0035116 and embryonic hindlimb morphogenesis and biological process this GO :0040008 and regulation of growth and biological process this GO :0043202 and lysosomal lumen and cellular component this GO :0043395 and heparan sulfate proteoglycan binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0045807 and positive regulation of endocytosis and biological process this GO :0045879 and negative regulation of smoothened signaling pathway and biological process this GO :0045880 and positive regulation of smoothened signaling pathway and biological process this GO :0045926 and negative regulation of growth and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0046658 and anchored component of plasma membrane and cellular component this GO :0050680 and negative regulation of epithelial cell proliferation and biological process this GO :0060422 and peptidyl-dipeptidase inhibitor activity and molecular function this GO :0060976 and coronary vasculature development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072111 and cell proliferation involved in kidney development and biological process this GO :0072138 and mesenchymal cell proliferation involved in ureteric bud development and biological process this GO :0072180 and mesonephric duct morphogenesis and biological process this GO :0072203 and cell proliferation involved in metanephros development and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :2000096 and positive regulation of Wnt receptor signaling pathway, glypican 3 |
| Identity: |
4451 |
| Gene: |
GPC3 |
More about : GPC3 |
| Long gene name: |
glypican 3 |
| Synonyms gene: |
SDYS |
| Synonyms: |
OCI-5 SGBS SGBS1 SGB DGSX |
| Synonyms name: |
glypican proteoglycan 3 |
| Locus: |
Xq26, 2 |
| Discovery year: |
1996-08-08 |
| GenBank acession: |
L47125 |
| Entrez gene record: |
2719 |
| Pubmed identfication: |
8589713 9787072 |
| RefSeq identity: |
NM_004484 |
| Classification: |
Glypicans |
| Havana BLAST/BLAT: |
OTTHUMG00000022448 |
| Locus Specific Databases: |
LRG_505 |