| Antigen detectable with this ELISA kit: |
Brain (GAD1), Glutamate Decarboxylase 1 |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
31ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Brain (GAD1), Brain (GAD1), Brain (GAD1), Brain (GAD1) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 1, The concentration of Glutamate Decarboxylase 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Glutamate Decarboxylase 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Brain (GAD1), Glutamate Decarboxylase 1 |
| Short name: |
Brain (GAD1) ELISA kit, Glutamate Decarboxylase 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
67kDa)) Enzyme-linked immunosorbent assay reagent, Brain (glutamate decarboxylase 1 (brain, Glutamate Decarboxylase 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
67kDa), CPSQ1 and GAD and SCP, GAD1 and IDBG-640815 and ENSBTAG00000007258 and 517552, GAD1 and IDBG-74724 and ENSG00000128683 and 2571, Gad1 and IDBG-177867 and ENSMUSG00000070880 and 14415, Plasma membranes, protein N-terminus binding, this GO :0004351 and glutamate decarboxylase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006520 and cellular amino acid metabolic process and biological process this GO :0006538 and glutamate catabolic process and biological process this GO :0006540 and glutamate decarboxylation to succinate and biological process this GO :0007268 and synaptic transmission and biological process this GO :0007269 and neurotransmitter secretion and biological process this GO :0008152 and metabolic process and biological process this GO :0009449 and gamma-aminobutyric acid biosynthetic process and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0016595 and glutamate binding and molecular function this GO :0016829 and lyase activity and molecular function this GO :0016831 and carboxy-lyase activity and molecular function this GO :0018352 and protein-pyridoxal-5-phosphate linkage and biological process this GO :0019752 and carboxylic acid metabolic process and biological process this GO :0030170 and pyridoxal phosphate binding and molecular function this GO :0042136 and neurotransmitter biosynthetic process and biological process this GO :0042493 and response to drug and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0047485 and protein N-terminus binding and molecular function this GO :0061202 and clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane and cellular component, this GO :0004351 : glutamate decarboxylase activity, this GO :0004351 : glutamate decarboxylase activity and also this GO :0005515 : protein binding and also this GO :0016595 : glutamate binding and also this GO :0016829 : lyase activity and also this GO :0016831 : carboxy-lyase activity and also this GO :0030170 : pyridoxal phosphate binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0047485 : protein N-terminus binding, this GO :0005515 : protein binding, this GO :0016595 : glutamate binding, this GO :0016829 : lyase activity, this GO :0016831 : carboxy-lyase activity, this GO :0030170 : pyridoxal phosphate binding, this GO :0046982 : protein heterodimerization activity, this GO :0047485 : protein N-terminus binding, glutamate decarboxylase 1 (brain |
| Identity: |
4092 |
| Gene: |
GAD1 |
More about : GAD1 |
| Long gene name: |
glutamate decarboxylase 1 |
| Synonyms gene: |
GAD |
| Synonyms gene name: |
67kD) glutamate decarboxylase 1 (brain, 67kDa) , glutamate decarboxylase 1 (brain |
| Locus: |
2q31, 1 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
2571 |
| Pubmed identfication: |
1549570 |
| Havana BLAST/BLAT: |
OTTHUMG00000044175 |