| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SIGLEC9 |
| Specificity: |
This antibody reacts specifically with SIGLEC9 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SIGLEC9 |
| Gene ID: |
27180 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is highly expressed in peripheral blood leukocytes (but not eosinophils), Siglec-1 mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, Siglec-7, Siglec-8, Siglec-9 is closely homologous to Siglec-7, The common sialic acids of mammalian cells are N-acetyl-neuraminic acid (Neu5Ac) and N-glycolyl-neuraminic acid (Neu5Gc), The sialic acid-binding immunoglobulin superfamily lectins, a type I membrane protein, are immunoglobulin superfamily members recognizing sialylated ligands, basophils and mast cells, bone marrow, but not in parenchymatous cells, designated siglecs or sialoadhesins, highly expressed in monocytes and resident blood cells, is selectively expressed on human eosinophils, liver, mediates inhibition of natural killer cell cytotoxicity, placenta and spleen, sinus-lining cells and reticulum cells, the gene encoding it maps to chromosome 19q13, where it regulates their function and survival, 41 in humans, Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: the selectins and the sialoadhesins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CD329 |
| Also known as: |
SIGLEC9 Polyclonal Antibody |
| Other name: |
Anti-SIGLEC9 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SIGLEC9 |
| Short name: |
Anti-SIGLEC9 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-sialic acid binding Ig-like lectin 9 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Plasma membranes, SIGLEC9 and IDBG-65664 and ENSG00000129450 and 27180, carbohydrate binding, this GO :0005515 and protein binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0045087 and innate immune response and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0030246 : carbohydrate binding, this GO :0030246 : carbohydrate binding, sialic acid binding Ig-like lectin 9 |
| Identity: |
10878 |
| Gene: |
SIGLEC9 |
More about : SIGLEC9 |
| Long gene name: |
sialic acid binding Ig like lectin 9 |
| Synonyms gene name: |
sialic acid binding Ig-like lectin 9 |
| Locus: |
19q13, 3-q13, 4 |
| Discovery year: |
2000-03-29 |
| GenBank acession: |
AF135027 |
| Entrez gene record: |
27180 |
| Pubmed identfication: |
10903842 |
| RefSeq identity: |
NM_014441 |
| Classification: |
CD molecules Sialic acid binding Ig like lectins V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000182894 |