| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HCN3 |
| Specificity: |
This antibody reacts specifically with HCN3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HCN3 |
| Gene ID: |
57657 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
HCN channels are expressed in the brain, HCN3 and HCN4 exhibit subcortical distribution mainly concentrated in the hypothalamus and thalamus, HCN3 contains a segment characterized by a series of positively charged amino acids at every third position, In the brain, The HCN family consists of four members (HCN1-4), The pacemaker properties of HCN channels contribute to spontaneous rhythmic activity in the brain and heart, This region designated S4 is likely to be the voltage sensor of the protein, cyclic nucleotide-binding channels (HCN) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides, each with a core transmembrane segment domain and a C-terminal 120 amino-acid cyclic nucleotide-binding domain motif, heart, respectively, thalamus and testis, Hyperpolarization-activated |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA1535 |
| Also known as: |
HCN3 Polyclonal Antibody |
| Other name: |
Anti-HCN3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HCN3 |
| Short name: |
Anti-HCN3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HCN3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
19183 |
| Gene: |
HCN3 |
More about : HCN3 |
| Long gene name: |
hyperpolarization activated cyclic nucleotide gated potassium channel 3 |
| Synonyms gene name: |
hyperpolarization activated cyclic nucleotide-gated potassium channel 3 |
| Locus: |
1q22 |
| Discovery year: |
2002-09-02 |
| GenBank acession: |
AB040968 |
| Entrez gene record: |
57657 |
| Pubmed identfication: |
16382102 |
| RefSeq identity: |
NM_020897 |
| Classification: |
Cyclic nucleotide gated channels |
| Havana BLAST/BLAT: |
OTTHUMG00000035872 |